section 3 - testing Flashcards
what is the method of action of EDTA tubes
chelates calcium to prevent coagulatoin
what is the method of action of sodium citrate tubes
binds calcium to prevent coagulation
- necessary to have full tube due to ratio of anticoag to blood
define PPP and when it’s used
platelet poor plasma
used for coagulation studies
define PRP and when it’s used
platelet rich plasma
used for platelet function studies
define IPF
immature platelet fraction
- reticulocytes of platlets
list disorders related to low IPF
- aplastic anemia
- leukemia
- BM suppression
- drugs
list disorderes related to increased IPF
- ITP
- TTP
- DIC
what does the PFA measure
- closure time -> replaced bleeding time tests
- tests primary hemostasis
how is platelet aggregation measured
aggregometry
what differentiates collagen as an aggregating agent from the rest
single phase with lag
list platelet aggregation agents
- adp
- epinephrin
- collagen
- thrombin
- arachidonic acid
- ristocetin
what differentiates Ristocetin as an aggregating agent from the rest
biphasic but measure platelet agglutination
- vWF binding Gp1b
- no shape change
describe vWF antigen (vWF:Ag)
determines which vWF multimers are present
describe vWF activity - ristocetin (vWF:RCo)
how well are platelets functioning
- pt plasma to agglutinate donor platelets in presence of Ristocetin
