SEC HEMO TESTS

Question 1

Measures the period required for free formation of
blood to clot after it has been removed from the body

Answer 1

Clotting Time

Question 2

Clotting time 2 methods

Answer 2

• Methods:
o Capillary Blood Method
▪ Slide drop method
▪ Capillary method (Dale and Laidlaw’s)
o Whole Blood (Lee and White Method)

Question 3

• Methods:
o Capillary Blood Method
▪ Slide drop method
□ Normal value:

Answer 3

• Methods:
o Capillary Blood Method
▪ Slide drop method
□ Normal value: 2-4 mins

Question 4

▪ Capillary method (Dale and Laidlaw’s)
o Whole Blood (Lee and White Method)
▪ Normal value:

Answer 4

7-15mins

Question 5

Monitors coagulation factors of the extrinsic and
common pathways

Answer 5

proth time

Question 6

Used to monitor oral anticoagulant therapy

Answer 6

proth time

Question 7

When______ is added to PPP along
with calcium, it reacts with factor VIIa to convert
factor X to Xa.

Answer 7

thromboplastin

Question 8

Factor Xa, along with factor Va, phospholipids,
and calcium, converts prothrombin to____.

Answer 8

thrombin

Question 9

o Standardized way of reporting PT results
o Introduced to minimize the difference in PT
results due to different reagent-instrument
combinations

Answer 9

INR - International Normalized Ratio

Question 10

Used to monitor heparin therapy (heparin -
antithrombin)

Answer 10

Activated Partial Thromboplastin Time (APTT)

Question 11

Stypven Time
aka?

Answer 11

Russell’s Viper Venom Time

Question 12

Reagent: of Stypven Time

Answer 12

East Indian Viper venom (Vipera russelli)

Question 13

Normal value Stypven time

Answer 13

6-10 secs

Question 14

Reptilase - thrombin-like enzyme from the reptile

which Converts fibrinogen to fibrin

Answer 14

Bothrops atrox

Question 15

Detects fibrinogen deficiency
• Unaffected by heparin therapy

Answer 15

Reptilase Time

Question 16

Reptilase differs from thrombin in which
reptilase cleaves ______, while
thrombin cleaves fibrinopeptides A and B

Answer 16

fibrinopeptide A

Question 17

Duckert’s Test
aka?
• Detects Factor XIII deficiency

Answer 17

• Aka 5M Urea Solubility Test

Question 18

Duckert’s Test reagent

Answer 18

1% monochloroacetic acid

Question 19

Principle of test; Patient’s plasma is clotted by the addition of
calcium chloride.

Answer 19

Duckert’s Test

Question 20

Factor XIII deficiency: clot is soluble to _____

Answer 20

5M urea

Question 21

Presence of Factor XIII: clot is insoluble to 5M urea for
____ hrs

Answer 21

24 hrs

Question 22

Mixing Studies
• Aka??

Answer 22

• Aka substitution studies

Question 23

A procedure done to identify specific factor
deficiencies by mixing correction reagents with a
patient’s plasma, and performing PT and APTT

Answer 23

mixing studies

Question 24

• Screening procedure for the measurement of
fibrinolytic activity

Answer 24

Euglobulin Clot Lysis Time

Question 25

• protein fraction that precipitates when
  plasma is diluted with water and acidified

Answer 25

• Euglobulin

Question 26

Addition of 1% acetic acid to diluted plasma
causes euglobulin fraction to precipitate.

Answer 26

Euglobulin Clot Lysis Time

Question 27

• Detects the presence of fibrin monomers by causing
the formation of fibrin strands or gel-like clots
(paracoagulate) - paracoagulation

Answer 27

Protamine Sulfate Test

Question 28

Reagent: Protamine sulfate
o Treatment for heparin overdose
o Warfarin overdose - Vit. K

Answer 28

Protamine sulfate test

Question 29

• Less sensitive but more specific test than protamine
sulfate test
• Detects the presence of fibrin monomers

Answer 29

Ethanol Gelation Test

Question 30

Reagent
o 50% ethanol
o Sodium hydroxide

Answer 30

Ethanol Gelation Test

Question 31

Used to evaluate the presence of specific fragments
arising from degradation of fibrin

Answer 31

Latex D-dimer test

Question 32

• Principle: Enzyme immunoassay and latex bead
o The test uses latex beads coated with
monoclonal antibody specific for D-dimer

Answer 32

Latex D-dimer test

Question 33

• Single test to differentiate primary and secondary
fibrinolysis.

Answer 33

latex d-dimer test

Question 34

o The test has no specificity for fibrinogen and,
therefore identifies plasmin action towards
fibrin.

Answer 34

latex d-dimer test