SDS-PAGE

Question 1

sodium dodecyl sulfate

Answer 1

a reagent the coats denatures proteins evenly in negative charge and prevents refolding after heat treatment : separation only based on size to native charge
SDS and reactive agent break disulfide bond
keeps protein denatured during electrophoresis

Question 2

polyacrylamide gel

Answer 2

polymer mesh to impede protein movement
reagents: APS/TEMED: initiates polyacrylamide formation
Bisacrylamide: cross-linkage and gel integrity
acrylamide: concentration depends on how large the proteins are: larger less smaller more

Question 3

electrophoresis

Answer 3

protocol uses electricity to separate charged particles

smallest will go further as less restricted by mesh of polyacrylamide gel

Question 4

SDS-PAGE

Answer 4

laboratory protocol often used to separate proteins by size often to asses purity or to isolate one protein of interest