sB1h enzyme activity Flashcards

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1
Q

describe the effect of temp on enzyme activity

A

increase temp - activity of the enzyme ( rate of reaction gets faster)
past the optimum - activity of enzymes go rapidly down to 0 because HIGH TEMP - enzymes vibrate - shape of active site changes,substrate doesn’t fit, active site denatured ( no longer catalyse the reaction)

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2
Q

effect of substrate concentration on enzyme activity

A

low substare concentration - free active sites
too much substrate - all active sites filled, no free active sites to increase the rate of reaction

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3
Q

effect of pH on enzyme activity

A

pH more acidic or alkaline - activity down to 0 ( active site denatures )

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4
Q

what is optimum pH temp of an enzyme

A

enzyme fastest possible rate, maximum frequency of collision between enzyme and substrate

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