SB1 : Key Concepts Flashcards
what is magnification?
-the process of enlarging the appearance/image of something
-how many times bigger the image looks to the real size
what is the real real size?
-the size of the specimen you put under the microscope
what is image size?
what you see when you look through the microscope
how do you calculate magnification?
magnification = image size
—————-
actual size
how do you calculate the total magnification?
-times the two magnifications together
what is resolution?
-the ability to distinguish between two points as separate structures
-the level of detail that can be seen
what are the steps for using a microscope?
1)carry to workspace, one arm on bottom and other on the arm
2)move the lenses to the ones with smallest magnification
3)carefully put your prepared slide on the stage
4)connect and turn on the microscope
5)look through the eyepiece lens and turn the curse focus wheel till you can see the specimen
6)use fine focusing wheel to make the image clearer
7)if you want a bigger image increase the magnification
8)observe and make a drawing
how do you prepare a microscope slide?
-take a thin slice (1 cell thick) from the outermost layer (epidermis)
-place carefully on the slide and push down
-add a stain (e.g. iodine)
-place a cover slip at an angle to eliminate air bubbles
-place slide on stage
what are the 3 main monomer/polymers ?
monomer 1: amino acids
polymer 1: proteins
monomer 2: glucose molecules
polymer 2: starch
monomer 3: fatty acids and glycerol
polymer 3: lipids
why does a calorimeter have a closed system?
- so no heat can enter or escape
why is a stirrer used for a calorimeter?
-to ensure that the heat is evenly distributed
what is the test for starch?
-iodine
-goes from orange -> blue/black
what is the test for reducing sugars?
-Benedict’s regent
-brick red- positive
-blue- negative
what is the test for protein?
-Biuret
-blue = none
-purple = posotive
what is the test for lipids?
-ethanol
-transparent = negative
cloudy = positive
