SAS 9 Flashcards
Simplest type of chromatography
Column chromatography
what is the other name for the stationary phase
adsorbEnt
what is the other name for the solvent
eluAnt
what is the other name for the product
eluATe
This is SPOTTING OF A MIXTURE of components at one end of an adsorbent-coated glass plate
Thin layer chromatography (TLC)
what is the stationary phase in Thin Layer Chromatography (TLC)
A thin plate of silica gel gypsum
what is the best solvent in TLC?
Benzene
or
Chloroform with 10% ethanol
This is the ratio of the distance traveled by the front of the mobile phase from the point of application of the test substance
Retention value
This is based on the partition phenomenon
Reverse Phase Chromatography
Uses as the mobile phase as inert gas called the carrier gas
Gas chromatography
What is the stationary phase in the GC?
liquid substrate
In RPC which is the stationary phase?
Non-polar solvent
in RPC which is mobile phase?
Polar solvent
what are the factors affecting GC separation?
Volality, Polarity, Column temperature, column length, column packing polarity, flow rate of gas
a.k.a Liquid-Solid chromatography
Adsorption chromatography