SARS-CoV-2

Question 1

4 structural proteins?

Answer 1

Spike S, Envelope E, Membrane M, Nucleocapsid N

Question 2

Nsp1

Answer 2

Mediates RNA processing and replication

NSP1 binds to 18S ribosomal RNA in the mRNA entry channel of the ribosome to interfere with the translation of mRNA

Question 3

Nsp2

Answer 3

Modulates survival signaling of host cell

Question 4

Nsp3

Answer 4

Separates translated proteins

Question 5

Nsp4

Answer 5

contains transmembrane domain 2 (TM2) and modifies ER membranes

Question 6

Nsp5

Answer 6

participates in the process of polyprotein during replication

Question 7

Nsp 6

Answer 7

presumptive transmembrane domain

Question 8

Nsp7 and Nsp8

Answer 8

significantly increased the combination of nsp12 and template-primer RNA
NSP8 and NSP9 binds to the 7SL RNA which locates at the Signal Recognition Particle to disrupt protein trafficking to the cell membrane

Question 9

Nsp9

Answer 9

functions as an ssRNA-binding protein
NSP8 and NSP9 binds to the 7SL RNA which locates at the Signal Recognition Particle to disrupt protein trafficking to the cell membrane

Question 10

Nsp10

Answer 10

critical for the cap methylation of viral mRNAs

Question 11

Nsp12

Answer 11

contains the RNA-dependent RNA polymerase (RdRp), which is a critical composition of coronavirus replication/transcription

Question 12

Nsp13

Answer 12

binds with ATP and the zinc-binding domain in nsp13 participates in the process of replication and transcription

Question 13

Nsp14

Answer 13

proofreading exoribonuclease domain

Question 14

Nsp15

Answer 14

has Mn(2+)-dependent endoribonuclease activity

Question 15

Nsp16

Answer 15

a 2’-O-ribose methyltransferase

Upon SARS-CoV-2 infection, NSP16 binds mRNA recognition domains of the U1 and U2 snRNAs to suppress mRNA splicing