Sanger Sequencing Mathod Flashcards by Samantha Jarvis | Brainscape

Q

Sanger

Primer annealing?

A

Primer anneals at 3’ end of template strand

- up to max of 20bp long

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Q

Sanger

Extension?

A

DNA polymerase assembles new DNA chains using four nitrogenous bases (dNTP)
DNA polymerase will not work without a primer

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Q

Sanger

Termination?

A

-reaction mixture contains fluorescent labelled dideoxyrinnucleic (ddNTP), miss hydroxyl group on third carbon, stops elongation

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Q

Sanger

Gel electrophoresis

A

DNA fragments are run through a gel according to size

- DNA fragments differ from one base pair, so gel is read from bottom to top

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Q

Sanger

Strand separation?

A

Double stranded DNA is separated into single strands using high heat

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