Sample Collection, Handling, Preservation, and Transport

Question 1

Most commonly submitted sample for parasite examinations

Answer 1

STOOL

Question 2

The most common procedure performed in area of parasitology

Answer 2

Examination of a stool specimen for ova and parasites (O&P)

Question 3

test for ova fecalysis is always ______

Answer 3

stool sample

Question 4

Fecal specimens should be collected in

Answer 4

clean, wide mouthed/watertight containers with tight-fitting
lids, waxed cardboard box (1/2 pint) or matchbox.

Question 5

_______ should not be allowed to contaminate the stool specimen

Answer 5

Urine

Question 6

Container used on basic fecalysis

Answer 6

Clean Container

Question 7

Container used on stool culture

Answer 7

Sterile Container

Question 8

Stool ___ be retrieved from toilet bowl water
because

Answer 8

Should not

Question 9

The typical stool collection protocol consists of _
specimens.

Answer 9

3

Question 10

one specimen collected every other day or a total of _ collected in _ days

Answer 10

3;10

Question 11

Other name for protozoan infection (severe diarrhea)

Answer 11

Amebiasis

Question 12

Stool samples from patients whose therapy includes barium, bismuth or mineral oil should be collected

Answer 12

prior to therapy or not until 5-7 days

Question 13

What element is taken for diarrhea

Answer 13

Bismuth

Question 14

Collection of specimens from patients who have taken antibiotics or antimalarial medications should be delayed for _ weeks

Answer 14

2

Question 15

Patients who have been treated for protozoan infections are typically checked __ weeks after therapy.

Answer 15

3-4

Question 16

A patient treated for helminth infection may be checked ___ weeks post therapy

Answer 16

1-2

Question 17

Taenia may be delayed for ___ weeks post therapy

Answer 17

5-6

Question 18

Routine stool examination requires a ____ sized/marble stool

Answer 18

Thumb

Question 19

formed stool or about ___
tablespoons (__ mL) of watery/diarrheic stool.

Answer 19

5-6;10

Question 20

Liquid specimens be examined within ___

Answer 20

30 minutes

Question 21

semi- formed specimens should be examined within ___ of passage

Answer 21

1 hour

Question 22

If a number of specimens are received at the same time, pick out the_____ stools

Answer 22

liquid;watery

Question 23

____ in soft or watery stools may possibly yield the presence of trophozoites

Answer 23

Blood and mucus

Question 24

By gross examination, tapeworm ____ and
adult nematodes may be found on the stool sample

Answer 24

proglattids

Question 25

Recovery of the ____ on the stool sample following therapy indicates success of treatment.

Answer 25

tapeworm scolex

Question 26

Feeding stage of protozoa

Answer 26

TROPHOZOITES

Question 27

Diagnostic stage of protozoa

Answer 27

TROPHOZOITES

Question 28

Non-feeding stage of protozoa

Answer 28

CYSTS

Question 29

Infective stage of protozoa

Answer 29

CYSTS

Question 30

A ___ collected stool sample, which is immediately submitted to the laboratory, is the ideal specimen for parasitic examination

Answer 30

freshly

Question 31

Are substances that preserve the morphology of protozoa and prevent further development of certain helminth eggs and larvae

Answer 31

Fixatives

Question 32

The ratio of fixative to stool

Answer 32

3:1

Question 33

The specimen must be fixed in the preservative for at least ___ before processing begins

Answer 33

30 minutes

Question 34

T or F: Temporary storage of fecal samples in a refrigerator
(4-8°C) may be acceptable.

Answer 34

T

Question 35

T or F: Never freeze stool samples nor keep them in incubators

Answer 35

T

Question 36

Refrigerated samples are only suitable for ___testing (CDC, 2016)

Answer 36

antigen

Question 37

An aqueous solution of formaldehyde, about 37-40%
weight in volume.

Answer 37

FORMALIN

Question 38

Recommended concentration of formalin for preservation of protozoan cyst

Answer 38

> 5%

Question 39

Recommended concentration of formalin for preservation of helminth eggs and larvae

Answer 39

> 10%

Question 40

Comprised of a plastic powder that acts as an adhesive for the stool specimen when preparing
slides for staining.

Answer 40

POLYVINYL ALCOHOL (PVA)

Question 41

Most often combined with Schaudinn’s solution, which usually contains zinc sulfate, copper sulfate or mercuric chloride as a base.

Answer 41

POLYVINYL ALCOHOL (PVA)

Question 42

Ecofriendly fixative (PVA)

Answer 42

ECOFIX

Question 43

Universal fixative, ecofriendly, contains no formalin, no PVA and no mercury.

Answer 43

TOTAL-FIX

Question 44

A combination of preservative and stain for fecal specimens, especially used in field surveys.

Answer 44

MERTHIOLATE-IODINE-FORMALDEHYDE

Question 45

For many years, considered as the “GOLD STANDARD”

Answer 45

SCHAUDINN’S SOLUTION

Question 46

Alternate to Schaudinn’s; contains 10% formalin as a fixative plus sodium acetate which acts as a buffer.

Answer 46

SODIUM-ACETATE FORMALDEHYDE

Question 47

The specimen should be placed into a _____ for transport to the laboratory.

Answer 47

Zip lock plastic bag

Question 48

10% Formalin (for wet mount)

Answer 48

1 part stool
3 parts formalin
Place in vial, crush stool thoroughly, preserves specimen indefinitely if bottle is closed

Question 49

MIF (for wet mount)

Answer 49

4.7 mL of MIF
0.3 mL of Lugol’s lodine in a vial/bottle (just before dispatching)
Add 2 mL of stool, crush with a glass rod/stick; preserves specimen indefinitely

Question 50

PVA (for permanent staining)

Answer 50

30 mL of PVA or 3/4 full in a bottle/vial
Enough fresh stool to fill the container
Break stool with a stick and cover, preserves all forms of parasites indefinitely