S4) Enzymes Flashcards
What are enzymes and what do they do?
Enzymes are protein catalysts that mediate metabolic pathways by increasing the velocity of chemical reactions while not being consumed during the reaction
Identicy 5 characteristics of enzymes
- Highly specific
- Unchanged after reaction
- Increase rate of reaction
- Proteins
- Does not affect reaction equilibrium
What is an active site?
An active site is the special cleft on an enzyme containing amino acid side chains that bind substrates and trigger catalysis
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Identify 4 characteristics of active sites
- Cccupies a small part of the enzyme
- Formed by amino acids
- Clefts/crevices
- Bind to substrates through weak bonds
Identify 2 different hypotheses which govern substrates binding to an enzyme
- Lock and key hypothesis – active sites have a complementary shape to the substrate
- Induced fit hypothesis – binding of substrates can induce chages in the comformation of the active site
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Describe the 5 steps involved in enzyme catalysis
- Substrate binds to active site on enzyme
- Conformational change occurs and ES complex forms
- Catalysis occurs
- ES complex is converted to EP complex
- EP compex dissociates to enzyme and product
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What is an allosteric site?
An allosteric site is a cleft on an enzyme where an activating/inhibiting molecule may bind, changing the shape of the enzyme and influencing its ability to be active
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Outline the specificity of enzymes
- Enzymes are highly specific, interacting with 1/few substrates and catalysing only one type of chemical reaction
- The set of enzymes made in a cell determines which metabolic pathways occur in that cell
Can enzymes be regulated?
Why is this needed?
Enzyme activity can be regulated (increased or decreased) so that the rate of product formation responds to cellular need
Why are many enzymes localised in specific organelles within the cell?
Compartmentalisation serves to:
- Isolate the reaction substrate/product from other competing reactions
- Provides a favourable environment for the reaction
- Organises thousands of cell enzymes into purposeful pathways
What is activation energy?
Activation energy is the minimum energy required by a chemical system to start a chemical reaction
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How do enzymes impact activation energy in a chemical reaction?
- Activation energy is usually very high, leading to slow rates in uncatalyzed chemical reactions
- Enzymes work to lower activation energy, so more particles are able to overcome the transition state and react
- Rate of reaction increases
![](https://s3.amazonaws.com/brainscape-prod/system/cm/223/503/822/a_image_thumb.png?1509103658)
What is the rate of reaction?
The rate of a reaction is the number of substrate molecules converted to product per unit time ( [product]/time )
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How does the rate of reaction vary in the presence of an enzyme?
The rate of an enzyme-catalysed reaction increases with substrate concentration until a maximal velocity (Vmax) is reached
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Why does the rate of reaction level off in enzyme-catalysed reactions?
The levelling off of the reaction rate at high substrate concentrations reflects the saturation of all available enzyme active sites with substrate
![](https://s3.amazonaws.com/brainscape-prod/system/cm/223/504/397/a_image_thumb.png?1509104286)
Identify and describe 2 factors which increases the rate of reaction
- Temperature: increases no. of molecules with activation energy
- Concentration: increases chance of molecular collisions