S2 - Human Nutrition Flashcards
biological molecules:
-carbohydrates
-proteins
-lipids
carbohydrates:
-they are made of carbon, oxygen and hydrogen
-starch and glycogen are large, complez carbohydrates, which are made up of many smaller units joined together in a long chain
maltose (and other simple sugars) -> starch
proteins:
-proteins are made up of long chains of amino acids
-they all contain carbon, nitrogen, hydrogen and oxygen atoms
amino acids -> proteins
lipids:
-lipids (fats and oils) are built from fatty acids and glycerol
-lipids contain carbon, hydrogen and oxygen atoms
glycerol & fatty acids -> lipid
how to make a food sample:
1) get a piece of food and break it up using pestle and mortar
2) transfer the ground up food to a beaker and add some distilled water
3) give the mixture a good stir with a glass rod to dissolve some of the food
4) filter the solution using a funnel lined with filter paper to get rid of the solid bits of food
testing for glucose:
use the Benedict’s Test to test for glucose:
-if the food sample contains glucose, the solution in the test tube will change from its normal blue colour
-it will become green or yellow in low concentrations of glucose
-it will become brick-red in high concentrations of glucose
testing for starch:
use Iodine solution to test for starch:
-if the sample contains starch, the colour of the solution will change from browny-orange to black or blue-black
testing for proteins:
use the Biuret Test to test for proteins:
-if the food sample contains protein, the solution will change from blue to pink or purple
-if no protein is present, the solution will stay blue
testing for lipids:
use the Sudan III Test to test for lipids:
-if the sample contains lipids, a milky white emulsion will form
-if the sample doesn’t contain lipids, it will remain colourless
practical: investigating starch production:
1) cover half of a small leaf with foil
2) place the plant on a windowsill for 48 hours so that ligh can reach it
3) put the leaf into boiling water to kill and preserve it
4) put the leaf in a boiling tube containing hot ethanol for 10 minutes (this removes the chlorophyll pigment)
5) dip the leaf in boiling water to soften it
6) put lead in a Petri dish and cover with iodine solution
7) the covered half of the leaf will remain orange-brown, whereas the exposed half will change to blue-black (as iodine solution changes colour in the presence of starch, as photosynthesis tuned the glucose into starch for storage)
nutrition in humans:
-humans need to eat a balanced diet in order to mantain their health
-this should consist of carbohydrates, proteins, lipids, dietary fibre, vitamins, minerals and water
nutrient sources and functions:
-carbohydrates: bread, cereals, pasta, rice, potatoes
-> a high energy source
-proteins: meat, fish, eggs, pulses -> for growth and repair
-lipids: butter, oil, nutes -> a high energy source and for insulation
-dietary fibre: vegetables, bran -> to provide roughage to keep food moving through gut-defiency causes constipation
-vitamin A: carrots, green vegetables -> needed for vision, especially in the dark, and for growth
-vitamin C: citrus fruits, broccoli, peppers -> helps to absorb iron
-vitamin D: margarine, oily fish -> helps to absorb calcium
-calcium: milk -> for bone and teeth strength-defiency can cause rickets (curving of bones)
-iron: red meat -> needed for haemoglobin-defiency can cause anaemia
-water: water, juice, milk -> needed for cell reactions to take place
factors affecting energy requirements: age
-energy requirements generally increases as we approach adulthood
-energy needs of adults go down as they age
factors affecting energy requirements: activity levels
-if you are more active then you will need more energy for movement
factors affecting energy requirements: pregnancy
-energy requirements will increase in order to support growth of foetus
-energy needs also increase due to the extra mass of the baby