RP2 Calculating Miotic Index Using Plant Cells

Question 1

Where in plant cells can undergoing mitosis be found?

Answer 1

Meristem tissue at shoot and root tips

Question 2

What is the mitotic index?

Answer 2

Ratio of cells undergoing mitosis to the total number of cells in sample.

Question 3

Outline the procedure to prepare a root tip slide.

Answer 3

1. Warm 1m HCI to 60°c in water bath
2. Cut a root tip using a carpel and add HCI. Leave for 5 minuets.
3. Remove HCI and wash with distilled water
4. Cut tip of root tip sample and place on a slide.
5. Add a few drops of stain to make chromosomes visible.

Question 4

State formula for mitotic index.

Answer 4

Mitotic index= number of cells with visible chromosomes/ number of cells in sample.

Question 5

State the hazards and precautions for reagents used in this procedure.

Answer 5

HCI- corrosive, avoid contact with skin, wear eye protection
Toluidine blue o stain- irritant, avoid contact with skin, wear eye protection.
Scalpel- cut away from fingers