RP11:test diabets/glucose In Urine.🥼

Question 1

Give me the test for diabetes with urine samples given to you.

Answer 1

1-Take a pair of goggles and a test tube rack with 4-6 test tubes
2-place test tube in the racks and name them accordingly A,B,C,D,E,F
And write initials
3-use different pipette to add 2cm cubed to each corresponding test tube
4-use different puppets to add 10 drops of HCI to earth test tube
5-use test tube holder to place them into water water bath of 90 degrees for 4-5 minutes.
6-after cool down test tube with cold tap water leave to cool
7-sodium hydrogenate carbonate (NaHCO3) powder until it stops fizzing
Can confirm the solution is alkaline by placing universal indicator in swells in Pasteur pipette
8-add 0.5cm3 of Benedict solution with pipette to test tube In each solution
9-heat the test solution to boiling point in water baths and heat for 2 minutes
10-observe the colour change over the 2 minutes

Question 2

Why do we add HCI?

Answer 2

To break glycosidic bond
To release galactose and glucose in sucrose and all monosaccharides which Benedict can react with

Question 3

Why do we use NaOH3 in solution?

Answer 3

So solution is alkaline because acids and acids don’t react but just get together ,it is needed so that Benedict reagent to work

Question 4

Give me the Hazard assessment.
(Urine)

Answer 4

Benedict reagent:irritate skin and eyes so we must wear goggles
-And if you got any on you wash eye /skin immediately with cold water
-Low risk
-hot liquid:water bath,can cause scalding so use tongs to remove the test tube rack
-And if any hot liquid got on you just rinse affected area on cold tap water.

Question 5

How to deter by colour the quantity of glucose in urine

Answer 5

Green:transable
Yellow:low
Orange:moderate
Brick red:high