RP11 - Measuring the concentration of glucose using a calibration curve

Question 1

How can benedicts solution be used to measure the concentration of glucose in solution

Answer 1

• Use colorimeter to measure the absorbance of a series of dilution series
• Compare the absorbance of an unknown sample to the calibration curve

Question 2

What are the axes in a calibration curve

Answer 2

Absorbance against glucose concentration

Question 3

How can you increase the accuracy of the estimate of unknown glucose concentrations

Answer 3

• Use smaller intervals