RP11 Flashcards

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1
Q

Describe how a calibration curve could be produced for glucose

A
  1. Use distilled water and a glucose solution of known
    concentration to produce a dilution series (of
    glucose solutions of known concentrations)
  2. Heat a set volume of each solution with a set
    volume of Benedict’s solution
  3. Measure absorbance (of light) of each solution
    using a colorimeter
  4. Plot a graph of absorbance (y axis) against
    concentration of glucose solution (x axis) and
    draw a line / curve of best fit
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2
Q

Describe how the concentration of glucose in an unknown ‘urine’ sample
can be identified using a calibration curve

A
  1. Perform Benedict’s test on sample using same volumes of solutions used in producing calibration curve
  2. Measure absorbance using a colorimeter
  3. Absorbance value for ‘urine’ sample read off calibration curve to find associated glucose concentration
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3
Q

Give examples of variables that should
be controlled

A

● Volume of sample used
● Volume of Benedict’s solution
● Temperature of water bath
● Time samples were heated for in water bath

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4
Q

Explain why a high blood glucose
concentration can cause glucose to be
present in the urine of a diabetic person.

A

● Not all glucose reabsorbed at proximal convoluted tubule
● As glucose carrier / cotransporter proteins are saturated /
working at maximum rate

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