RP. pH and Enzymes Flashcards

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1
Q

Describe how you would investigate the effect of pH on the rate of reaction of the amylase enzyme

A

Place starch solution, amylase solution and buffer solution in a water bath at 25°C for a few minutes

● Combine solutions and add a drop of the mixture to iodine solution on a tile

● Add the mixture to iodine solution drops on the tile at fixed 30s intervals until

the iodine solution remains brown (does not turn blue-black)

● Calculate the rate of reaction using 1 / time taken for solution to remain brown

● Repeat at different pH values using different buffer solutions

● Plot graph of rate (y) against pH (x)

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2
Q

Which enzyme catalyses the breakdown of starch in the human digestive system?

A

Amylase - catalyses breakdown of starch to maltose

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3
Q

Why must samples be left in the water bath before solutions are mixed?

A

To allow temperature to equilibrate

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4
Q

What is a buffer solution?

A

A solution which can resist changes in pH

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