RP 1 Flashcards
Factors that control rate of enzyme reaction.
-enzyme conc
-initial subs conc
-pH
-temp
Controls for experiment on enzyme controlled reaction.
-volume/conc of substrate/enzyme
-one test tube with boiled enzyme
Answering increase in rate questions.
1.more ESC form
2.more freq success collissions
3.plateaus subs/enzyme is used up
OR denatures due to change in shape of AS
Why you left test tubes for 10 mins.
1.so test tubes reach temp of water baths
Explain why you set up 3 exp with each temperature. How would you improve?
1.can get an average and spot anomalies
2.carry out 5 intervals and get a mean
You observed colour change , what 2 other methods that are more accurate.
1.colorimeter to measure absorbance
2.record time taken
3.filter/dry/weight ppt
How you ensure exp is fair for all temperatures and avoid errors?
1.measure same volume for amylase in each trial and starch conc for each temp
Why use a water bath?
1.test tube reach desired temperature of the water bath
Safety?
-water bath is hot, wear gloves
State one control variable that should be maintained constant on effect of pH on the rate of an enzyme-controlled reaction using amylase and starch. [1 mark}
-temperature
-test tube without buffer
-to see the effect of X on normal pH
Increasing reliability ?
-diff pH , diff temps
-repeat to get an average
-remove anomalies
Suggest a reason for the decrease in enzyme activity at extreme pH values. [2 marks]
-too high pH causes enzyme to denature
Suggest a reason for the decrease in enzyme activity at extreme pH values. [2 marks]
-too high pH causes enzyme to denature
Change that would ensure more awater lost or more o2 released if faster time?
1.use bigger chip or X
2.increase
3.increase temp
3.change pH
