rodaks Flashcards
often preferred to the powdered form because it mixes more easily with blood.
Liquid tripotassium EDTA
echinocytic red blood cells [RBCs], spherocytes, necrobiotic leukocytes, and vacuolated neutrophils. room temp at 5hours unacceptable
blood cell artifacts
Some patients’ blood under- goes an in vitro phenomenon called _______ when anticoagulated with EDTA
platelet satellitosis
occurs when platelet agglutinates are similar in size to WBCs and automated analyzers cannot distinguish the two.
Pseudoleukocytosis
counted as WBCs instead of platelets.
platelet clumps
probably the easiest to master. It is the most convenient and most commonly used method for making peripheral blood films.
Manual wedge technique
used for staining peripheral blood films and bone marrow smears. These are considered polychrome stains be- cause they contain both eosin and methylene blue
Pure Wright stain or a Wright-Giemsa stain (Romanowsky stain)8
also contain methylene blue azure
Giemsa stain
staining has been performed over a sink or pan with a staining rack. Slides are placed on the rack, film side facing upward
Manual technique.
common when a leukemia slide is placed on an automated slide stainer.
Understaining
are fast and easy. The whole process takes about 1 minute.
Quick stains
convenient and cost effective for low-volume laboratories, such as clinics and physi- cian office laboratories, or whenever rapid turnaround time is essential.
Quick stains
if they are present, the specimen should be rejected and another one should be collected.
presence of fibrin strands
popular digital cell morphology system.11 It automatically identifies optimal examination areas on a slide, then locates and captures focused images of the cells.
CellaVision
assessment of cell size
Redbloodcellmorphology.