RNA processing/Splicing

Question 1

What does mature eukaryotic mRNA consist of?

Answer 1

1) 3’ poly(A) tail
2) coding (translated) sequence in the middle
3) noncoding (untranslated) sequence on both sides of coding sequence
4) exons
5) 5’ cap

Question 2

What does mature prokaryotic mRNA consist of?

Answer 2

1) 5’ phosphate cap

2) several coding sequences separated by noncoding sequences

Question 3

What does pre-mRNA consist of in eukaryotes?

Answer 3

introns and exons

Question 4

What is the CTD of RNA polymerase II?

Answer 4

C terminal domain

Question 5

pre-mRNA processing steps

Answer 5

1) addition of cap
2) 3’ cleavage and addition of poly(A) tail
3) intron excision, exon ligation

Question 6

what is the role of introns?

Answer 6

Provides genomic “space” to allow shuffling of gene “chunks” that encode
individual protein domains to get genes that encode new combinations of domains