RNA processing

Question 1

important components for initiation of eukaryotic transcription

Answer 1

TATA box
transcription factors
RNA polymerase

Question 2

what is the TATA box?

Answer 2

DNA sequence within the promotor region of DNA

non-template nucleotide sequence box
approximately 25 nucleotides upstream from transcriptional start point

Question 3

what are transcription factors?

Answer 3

proteins that bind to the DNA and influence transcription

Question 4

role of transcription factors

Answer 4

one TF recognises the TATA box and binds
additional TFs recognise the bound TF and bind

RNA polymerase recognises TF
enables it to bind to DNA in correct position and orientation

Question 5

transcription initiation complex

Answer 5

TF bound to TATA box
additional TFs bound to TF
RNA polymerase binds to TF
addition TFs bind to DNA with RNA polymerase

DNA double helix unwinds
synthesis begins at the start codon