Rheumatoid Arthritis Flashcards
What is Rheumatoid Arthritis?
- chronic inflammatory disease with possible periods of remission
- symmetrically affects peripheral joints
- immune system-mediated -> AUTOIMMUNE DISEASE
- systemic disease -> there are often co-morbidities with it
What other co-morbidities can occur with Rheumatoid Arthritis?
- can potentially affect the eyes, lungs, heart, NS, spleen, lymph system and blood cells
- ie if feet are on “fire” might not want to exercise, become overweight therefore more at risk for HTN or heart failure or COPD…
- why it is always important to ask about all other symptoms too (secondary complications)
Autoimmune Disease
body’s immune system attacks and destroys healthy body tissue by mistake:
cellular immunity- tcells
humoral immunity- antibodies
these act in positive feedback together
Signs of Rheumatoid Arthritis
- early onset- swelling around the knuckles
- inflammation of the episclera (protective lining covering sclera or “white” of eye)
- RA nodules
- RA joint swelling
Epidemiology of RA
- 1 in 100
- can occur as any age (most prevalent= 25-50)
- female to male is 3:1
- global variation in prevalence (likely genetic driven)
- prevalence is growing (our society is obese as a whole which adds to the diagram)
Mortality of RA
- mortality is 30% higher than in the general population with the same age and sex
- if you have RA you have a 30% higher risk of dying than someone who doesn’t
- risk increases as you age (more immobile, more prone to more diseases)
Etiology of RA
- the exact cause(s) is unknown but it is know to involve:
1) Genetics (non-modifiable) - twin condordance 15-35% therefore external factors also likely involved; may predispose an indiv as a result of an enviro triggers
2) Smoking (known modifiable risk factor)
3) Infection (inconclusive)- may activate inflammatory pathways that “prime” the development of RA; driving risk factor b/c it drives the IS (i.e. a bacterial or viral infection- Epstein Barr Virus)
4) Autoimmunity
Explain how autoimmunity is involved in the etiology in RA.
-Ag-driven Ab’s [RF is an Ab detected in blood of 80% of adults with RF; anti-CCP - as sensitive of RF and more specific]
-ANA is a general test used to evaluate a person for autoimmune disorders such as lupus, MS, Sjogren’s syndrome, and RA
ESR reflects degree of inflammation in the body
-CRP also denotes inflammation but is a better indication of the amount of inflammation in the body than ESR
*first 2 are specific to RA, other 3 are general (if RA isn’t known yet, you can use the general test first and then go into specifics if you suspect RA)
Synovial Joint
- joins bones with a fibrous joint capsule that is continuous with the periosteum of the joined bones, constitutes the outer boundary of a synovial cavity, and surrounds the bones’ articulating surfaces
- the synovial cavity is filled with synovial fluid
- joint capsule is made up of an outer layer, the articular capsule, which keeps the bones together structurally, and an inner layer, the synovial membrane, which seals in the synovial fluid
Normal Synovial Membrane
thin layer of connective tissue (1-3 cell layers thick) between the joint capsule and synovial cavity
Normal Synovial Fluid
- an ultra-filtrate of blood that diffuses across the synovial membrane and into the joint cavity
- composed of hyaluronan and lubrican
What does hyaluronan do in the normal synovial membrane?
regulates cartilage viscosity
What does lubrican do in the normal synovial membrane?
lubricates surface of cartilage
What does fibronectin do in the normal joint?
- it is a general cell adhesion molecule
- acts like “glue to hold collagen cells together (like the cross-meshing that gives it the strength)
What do synoviocytes do in a normal joint?
- they are fibroblast-like
- are located on the inner surface of the capsule
- produce synovial fluid
RA Synovial Membrane
- the synovium has a greatly expanded expanded lining layer (8-10 cells thick) composed of activated cells and a highly inflammatory interstitial (Bcells, Tcells, macrophages, and vascular changes (thrombosis and neovascularization)
- pannus formation causes loss of bone and erosion of cartilage (hallmark characteristic of RA)
- the synovial fluid houses the activated inflammatory IS activated cells
- destruction of synoviocytes leads to the bone on bone
- key pathologic hallmarks seen peripherally in joint spaces in RA
- *in RA, self/non-self confusion results in destruction of synovial tissue
pannus
- thickened cellular membrane of fibrovascular tissue that invades the underlying cartilage bone
- at sites where synovium and cartilage are contiguous, RA synovial tissue (AKA PANNUS) invades and destroys adjacent cartilage and bone
Inflammation of the Synovium
- your immune system attacks your synovium (lining of membranes that surrounds your joints)
- immune system activated cells
a) fibroblast-like synoviocytes
b) macrophage-like synoviocytes
c) macrophages
d) t and b cells - mediators of inflammation enter the joint via the ultra-filtrate of blood that diffuses across the synovial membrane and into the joint cavity (blood vessels supply the synovial membrane)
Neutrophils
- recruited to the rheumatoid cavity
- in an activated state, releasing free radicals that damage hyaluronic acid and inactivate endogenous inhibitors of proteases, thus promoting damage to the joint
Osteoclasts
- responsible for bone resorption
- bone resoprtion= breaking down of the bone
- their formation is stimulated by the immune response
Fibroblast
- formation is stimulated by the IS, causes:
a) pannus formation leading to loss of cartilage and bone
b) matrix metalloproteinases –> degrade bone matrix
c) pro-inflammatory cytokines (tumor necrosis factor alpha, interferon gamma, and interleukin 6)
Pathogenesis of the Inflammatory Response
(see slide 13)
- mechanism involves both humeral and cellular immunity
- the processed antigen is recognized by major histocompatibility complex proteins on the lymphocyte –> activates it to stimulate the production of T and B cells
- APCs present Ag to Tcell to activate the Tcell, cause its proliferation, and secretion of cytokines (IL-17)
- activated Bcells produce plasma cells which produce self-ag’s (RF and ACPA)
- macrophages are responsible for the major damage to bone/synovium (major producers of pro-inflammatory cytokines)
Tcells and RA
- Tcells make up 30-50% of lymphocytes in the RA synovium
- activation by some auto-antigen leads to:
a) production of prostaglandins, cytotoxins, and cytokines
b) release of inflammatory cytokines, matrix metalloproteinases, osteoclasts, and Bcells - this activation requires 2 steps:
1) presentation of an APC to the Tcell receptor
2) CD28 (on T cell) binds to CD80/CD86 (on APC) - “co-stim” (CD28 acts as on switch)