Review Sheet for Basic Lab Skills Flashcards

1
Q

Micropopettor P20

A

Purpose: Accurately measure volumes less than 20 uL
Range measured:2-20uL
Maximum allowed: 20 uL

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2
Q

Micropipettor p10

A

Purpose: Accurately measures less than 10 uL
Range measured: 1-10 uL
maximum Allowed: 10uL

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3
Q

Hot Plate/Stirrer

A

Purpose: to heat chemicals solutions and mix sing stir bar
Range Measured: N/A
Maximum allowed: N/A

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4
Q

Micropipettor p200

A

Purpose:Accurately measure volumes less than 200uL
Range Measured:20-200uL
maximum Allowed:200uL

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5
Q

Micropipettor 1000

A

Purpose:Accurately measure volumes less than 1000uL
Range Measured:100-1000uL
Maximum Allowed:1000uL

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6
Q

Beaker

A

Purpose: Holds liquids
Range Measured: N/A
Maximum Allowed: N/A

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7
Q

Flasks

A

Purpose: Holds Solutions
Range Measured: N/A
Maximum Allowed/ N/A

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8
Q

Graduated Cylinders

A

Purpose: Measures out liquids
Range Measured: 5-1000mL
Maximum Allowed: 1000mL

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9
Q

Water Bath

A

Purpose: Heats up liquids
Range Measured: N/A
Maximum Allowed: N/A

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10
Q

Pipet aides

A

Purpose: Measures the solutions
Range Measured: 0.1-100mL
Maximum: Allowed:100mL

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11
Q

Bunsen Burners

A

Purpose:a small adjustable gas burner used in laboratories
Range Measured: N/A
Maximum Allowed: N/A

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12
Q

Vortexer

A

Purpose: Used more mixing solutions
Range Measured: N/A
Maximum Allowed: N/A

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13
Q

Heat block

A

Purpose: Heats up samples
Range Measured: N/A
Maximum Allowed: N/A

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14
Q

Incinerator

A

Purpose: Kills Bacteria
Range Measured: N/A
Maximum Allowed: N/A

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15
Q

Buret

A

Purpose: used in quantitative chemical analysis to measure the volume of a liquid or a gas
Range Measured: 1-50mL
Maximum Allowed: 50mL

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16
Q

Microcentrifuge Tube

A

Purpose: Spins the Liquids
Range Measured: N/A
Maximum Allowed: N/A

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17
Q

Electronic Balance (Red)

A

Purpose: Measures out the exact amount you need
Range Measured: N/A
Maximum Allowed: N/A

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18
Q

Electronic Balance(0.01)

A

Purpose: Measures masses of samples
Range Measured: 0.01g
Maximum Allowed: 0.01g

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19
Q

Electronic Balance(0.001)

A

Purpose: Measures masses of samples
Range Measured: 0.001g
Maximum Allowed: 0.001g

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20
Q

Incubator

A

Purpose: grows the bacteria
Range Measured: N/A
Maximum Allowed: N/A

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21
Q

Parafilm

A

Purpose: To sill of the bacteria
Range Measured: N/A
MAximum Allowed: N/A

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22
Q

Microcentrifuge tube

A

Purpose: processing and storage of small volume samples, extracts, and reagents
Range Measured: N/A
Maximum Allowed: N/A

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23
Q

15 mL conical tube

A

Purpose: Tubes used to hold liquids
Range Measured:15mL
Maximum Allowed: 15mL

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24
Q

50 mL conical tube

A

Purpose: Tube used to hold liquids
Range Measured: 50mL
Maximum Allowed: 50mL

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25
Q

1 mL Serological pipets

A

Purpose: Measures out the accurate amount of the solutions
Range Measured: 1mL
Maximum Allowed: 1mL

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26
Q

5 mL Serological pipets

A

Purpose:Measures out the accurate amount of the solutions
Range Measured: 5mL
Maximum Allowed: 5mL

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27
Q

10 mL Serological pipets

A

Purpose: Measures out the accurate amount of the solutions
Range Measured: 5mL
Maximum Allowed: 5mL

28
Q

25 mL serological pipets

A

Purpose: Measures out the accurate amount of the solutions
Range Measured: 25mL
Maximum Allowed: 25mL

29
Q

Peri dish

A

Purpose: Used for making agar plates
Range Measured: N/A
Maximum Allowed: N/A

30
Q

Eye Wash

A

Purpose: Cleans out the chemicals that go into your eyes
Range Measured: N/A
Maximum Allowed: N/A

31
Q

Inoculation loop

A

Purpose: Used for Streaking Bacteria
Range Measured: N/A
Maximum Allowed: N/A

32
Q

Body drench

A

Purpose: Cleans the chemicals off your body
Range Measured: N/A
Maximum Allowed: N/A

33
Q

Spectrophotometer

A

Purpose: Measures the intensity of light
Range Measured: N/A
Maximum Allowed: N/A

34
Q

Spatula

A

Purpose: mixing, scraping, and other tasks related to transferring materials and samples from one place to another.
Range Measured: N/A
Maximum Allowed: N/A

35
Q

pH meter

A

Purpose: Determines if something is an acid, base, or neutral
Range Measured: 1-14
Maximum Allowed: 14

36
Q

Caustic

A

able to burn organic tissue by chemical action

37
Q

Toxic

A

can harm or kill if swallowed

38
Q

flammable

A

can catch fire if not used right

39
Q

Chronic

A

continuous or repeated contact with a toxic substance over a long period of time

40
Q

Acute

A

chemicals that may be fatal as a result of a single exposure or exposure of a short duration.

41
Q

Flash point

A

the temperature at which a particular organic compound gives off sufficient vapor to ignite in air.

42
Q

FDA

A

Food and Drug Administration

43
Q

EPA

A

Environmental protection agency

44
Q

OSHA

A

Occupational safety and health administration

45
Q

NFPA

A

National Fire Protection Association

46
Q

Fine adjustment knob

A

Fine focus the image when viewing at a higher magnification

47
Q

Coarse adjustment knob

A

allows quick focusing by moving the object lens/stage up and down

48
Q

Coaxial adjustment knob

A

allows the user to change between the knobs by feel

49
Q

ocular lens

A

magnifies the image produced by the microscopes objective

50
Q

objective lens

A

unit that determines the basic performance/function of an optical microscope

51
Q

DI water (dH2O)

A

Removal of Ions

52
Q

Immersion Oil

A

increases the resolving power of the microscope by replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction.

53
Q

Analyte

A

A substance whose chemical constitution are being identified or measured

54
Q

Titrant

A

A Substance that is added to Titration

55
Q

Titration

A

Solution of known concentration is used to determine te concentration of an unknown soluton

56
Q

PPE

A

Personal Protection Equipment

57
Q

Aseptic technique

A

Using practices and procedures to prevent contamination from Pathogens

58
Q

Acidic

A

Having properties of and acid

59
Q

Alkaline

A

having properties of an alkali/pH greater than 7

60
Q

Hydroxide

A

compound of a metal with the group -OH

61
Q

Concentrated to Dilute

A

prepare 1000mL of 0.5 HCI using a 1.0 M HCI
(1.0)(V1)=(1000)(0.5)
V1=500

62
Q

Ratio Dilution

A

The urinalysis test requires volumes of 1mL. You need to dilute the samples 1:2, 1:5, and 1:10 9Urine water). What are the volumes 9mL0 of the urine and the volume of the water?
Vf/Df-Vc

63
Q

% solution

A

a 10% bleach solution of 500 mL is nned. What is the volume (mL) of bleach that you need to add to make the solution
(x/100)x(Vf)=ms

64
Q

Molar Solution

A

We need 1000mL of a 2.5 M salt solution. The molecular weight of the salt is 75g. What is the mass (g) that you need to add?
M=ms/M.W

65
Q

Serial Dilution

A

1:1,1:10,1:100,1:1000,1:10,000