Review Flash Cards - Biothechnology
What is the type of restriction endonuclease useful on biotechnology and why?
Type II because cut at defined region at recognition site.
During electrophoresis why do we have to place the DNA at the negative electrode and why is that?
Because DNA is negatively charged because it many phosphate groups that are strongly negatively charged.
What are the steps for inserting plasmid vectors?
Digestion: vector and DNA cut by same enzyme / Ligation: DNA inserted in plasmid and fused by ligase / Transformation: Plasmid transferred to bacteria
What is the difference between a selectable and differential marker?
Selectable: include gene for resistance to an antibiotic / Differential: include gene with restriction site in the gene so the gene is not correctly expressed if plasmid has new DNA
What is the difference between a genomic and cDNA library?
Genomic: DNA fragments that make up the full-length genome (introns&exons) / cDNA: DNA fragments encoded for proteins (exons)
