Revertants & Suppressor Mutants Flashcards
1
Q
What is a revertant mutant?
A
A mutant with two mutations such that one mutation reverses the effect of the other, restoring the phenotype to pseudo-wildtype.
2
Q
What is a suppressor mutant?
A
A mutant with two mutations that counteracts the first mutation by protein interaction.
3
Q
What is the Ames test for?
A
Determining chemical compound mutagenicity.
4
Q
How does it work?
A
Two cell plates with histidine-requiring Salmonella, on minimal media.
On one plate, a filter disk with buffer is applied. On the other, a filter disk with the testing chemical.
Random revertants will be visible on the ‘buffer plate’.
The number of revertants on the ‘chemical plate’ gives an indication of it’s mutagenicity.
