REVERSE BLOOD TYPING Flashcards
This method is the “confirmatory procedure”
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPING
What is the principle of Reverse/Indirect/Backward/Serum Grouping?
To detect unknown antibodies in the patient’s serum using laboratory prepared known red cell suspension
To detect unknown antibodies in the patient’s serum using laboratory prepared known red cell suspension. What method does this principle apply to?
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPING
What is the sample used in Reverse/Indirect/Backward or Serum Grouping?
Patient’s serum
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPING
Patient’s serum is preferred over plasma fibrinogen of plasma can cause spontaneous in vitro agglutination (false positive)
TRUE OR FALSE
TRUE
What is the reagent used for REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPING?
Laboratory prepared known RCS (2-5%) volume by volume concentration
of RCS
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPING
In practice, RCS prepared can only be stored for up to how many hours at what temp?
24 hours at refrigerator temp
Also used as control in reverse typing
Known O
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPIN
Known O should always having positive reaction
TRUE OR FALSE
negative reaction
FALSE
REVERSE/INDIRECT/BACKWARD OR
SERUM GROUPIN
Known O should always having positive reaction
TRUE OR FALSE
negative reaction
FALSE
Known AB is not essential/prepared in the lab
TRUE OR FALSE
TRUE
Reverse Typing: Test Tube Method
Weak reactions will be enhanced if the tubes are left for less than five
minutes
True or Fa;se
MORE THAN 5 mins
FALSE
Avoid excessive shaking which may
disperse weak agglutination.
True or False
TRUE
Weak reactions will be enhanced if the tubes are left for more than five
minutes room temperature before centrifugation
True or False
TRUE
The serum should NEVER be inactivated to circumvent this problem, unless only that portion to be used in this procedure is removed and heated at 56°C.
TRUE OR FALSE
TRUE
