Retroviruses

Question 1

What are retroviruse genomes?

Answer 1

ssRNA, enveloped and 8.5kb

Question 2

What cells can the replicate in?

Answer 2

Dividing cells

Question 3

What gene did retroviruses intergrate into?

Answer 3

LMO-2

Question 4

How many patients got leukaemia?

Answer 4

5/20 one died

Question 5

What is first stage of making a viral vector?

Answer 5

Make a packaging cell line

Question 6

What is second stage of making a vector?

Answer 6

Remove viral genes

Question 7

What could lentiviruses target?

Answer 7

Parkinsons Disease

Question 8

How can overcome random intergration?

Answer 8

Site specific recombination. Host genome has pseudo-attB and vector can encode for attP. Bacteria and phage model.Too big for retroviruses

Question 9

What are 1st generation Adeno vectors?

Answer 9

E1 gene deletion didnt work

Question 10

What are second generation adeno vectors?

Answer 10

No E1, E2 or E3.

Question 11

What are gutless vectors?

Answer 11

No viral genes just packaging and ITR and therapeutic gene.

Question 12

What disorder was being treated when someone died?

Answer 12

Ornithine transcarbamylase (OTC)

Question 13

What does E1B-55K do?

Answer 13

Inhibit p53