Retina

Question 1

Which stain is used in this slide?

Answer 1

Toludine blue

Question 2

What is the first layer of the retina?

Answer 2

Pigment epithelium - simple cuboidal epithelial cells with brown melanin granules

Question 3

What is the second layer of the retina?

Answer 3

Rods and cones - blue parallel strucures, thin - rods, thick - cones

Question 4

What is the third layer of the retina?

Answer 4

Outer limiting membrane - (very thin - not seen) blue barrier line, formed by peripherial processes of the Müller glial cells

Question 5

What is the fourth layer of the retina?

Answer 5

Outer granular (nuclear) layer - very wide layer, densly packed with small dark nuclei of rods and cones

Question 6

What is the fifth layer of the retina?

Answer 6

Outer plexiform layer - first synaptic layer, pale region

Question 7

What is the sixth layer of the retina?

Answer 7

Inner granular (nuclear) layer - nuclei of the bipolar cells (and horizontal cells, amacrine cells, and Müllerian glial cells), thinner than the outer granular layer - but larger nuclei

Question 8

What is the seveth layer of the retina?

Answer 8

Inner plexiform layer - second synaptic layer, pale region

Question 9

What is the eigth layer of the retina?

Answer 9

Ganglion cell layer - hardly identifible because there are only a few large but pale ganglion cells, usually one row only (forms the optic nerve). Nucleus and nucleolus may also be seen

Question 10

What is the night layer of the retina?

Answer 10

Optic nerve fibres - seen as dark blue structures around the ganglion cells. Capillaries are also seen (from central retinal arteries and veins)

Question 11

What is the tenth layer of the retina?

Answer 11

Inner limiting membrane - (thin line) innermost part of vitreous body, made by the inner processes of the Müller glial cells.