Reticulocyte

Question 1

How many days does reticulocytes stay on BM

Answer 1

2-3 days

Question 2

How many days does reticulocytes stay in the circulation

Answer 2

1 day

Question 3

Cytoplasmic inclusion co precipitate with —–

Answer 3

Mitochondria and ferritin masses

Question 4

What is the color to form visually seen on stain

Answer 4

DARK BLUE clusters with supravital stain

Question 5

Ferritin

Answer 5

Iron store

Question 6

Hair like fragments that appears web like

Answer 6

RETICS

Question 7

What has no remnants

Answer 7

RBC

Question 8

A dye that stains living cell and its inclusion

Answer 8

Supravital stain

Question 9

What are the supravital stain

Answer 9

NEW METHYLENE BLUE
BRILLIANT CRESYL BLUE

Question 10

Why do we perform retic ct

Answer 10

To measure the erythropoietic ability of the BM

Question 11

Measure the erythropoietic ability of the BM

Answer 11

In cases of anemia
BM to respond for anemia

Question 12

Ratio

Answer 12

1:1

Question 13

NV FOR NEWBORNS

Answer 13

4-6%

Question 14

Nv for adults

Answer 14

0.5-2.5 %

Question 15

Supravital stains should be filtered

Answer 15

Daily or before use

Question 16

Sample

Answer 16

Rbc mixed with edta
Capillary blood - for babies

Question 17

Procedure used in reticulocyte ct

Answer 17

Dry preparation

Question 18

Temperature for incubation for retic ct

Answer 18

Room temp

Question 19

How many mins should retics be incubated

Answer 19

10-15 mins

Question 20

Type of smear prepared

Answer 20

Wedge smear

Question 21

Objective used in viewing retics

Answer 21

100x objective

Question 22

Appearance of mature rbcs

Answer 22

Light to medium green without granules

Question 23

Appearance of reticulocyte

Answer 23

Light green with granules that stain deep blue

Question 24

Count the number of retics in ….

Answer 24

1000 red cells

Question 25

One oil immersion showing both mature erythrocyte and reticulocyte

Answer 25

Routine light microscopy method

Question 26

One oil immersion showing both red cells and reticulocyte

Answer 26

Miller disk method

Question 27

Inserted in the eyepiece

Answer 27

Miller ocular disk

Question 28

How many squares is counted on miller disk method

Answer 28

2 squares

Question 29

Counted in A OR B squares in miller disk

Answer 29

A- for cting retics
B- for RBCS

Question 30

How many ct of rbcs are counted in susceptible fields

Answer 30

12 rbcs

Question 31

Increased

Answer 31

• blood loss
• crises ass w/ hemophilic anemia
• subsequent for pernicious anemia

Question 32

Hallmark of anemia

Answer 32

Increased retic count

Question 33

Accuracy of counting

Answer 33

2 laboratorians are required to count the retics

Question 34

Result should agree at what percent

Answer 34

20%

Question 35

Poor drying (atmospheric moisture)

Answer 35

Refractile bodies

Question 36

May resemble dark blue clusters

Answer 36

Precipitates

Question 37

Howell jolly bodies

Answer 37

1-2 deep purple colored dense structure

Question 38

Heinz bodies

Answer 38

Light blue- green, periphery of rbc

Question 39

Pappenheimer bodies

Answer 39

Small iron clusters

Question 40

Color of pappenheimer bodies

Answer 40

Prussian blue

Question 41

Severe anemia

Answer 41

Proportion of dye to blood should be adjusted

Question 42

Miller disk

Answer 42

Inverted L