Results Flashcards
1
Q
Why is HT2 exposure greater?
A
Two possible reasons broadly. It was expressed to a greater degree or is more accessible to the antibody.
2
Q
Why did the tagging reduce titres?
A
Optimisation needed.
3
Q
Why did HT1 have such a negative effect?
A
- Interfered with activation
- Interfered with fusion
- Altered membrane trafficking.
4
Q
ANOVA and Dunnet’s
A
The data means were assumed to follow a normal distribution. ANOVA avoids cumulative type 1 errors.
Dunnet’s chosen as it compares each mean to a control mean.
Tukey compares all means to each other.
5
Q
Would any other statistical tests have been appropriate?
A
No
6
Q
Why no positive control for the Ig staining?
A
Couldn’t find one.
7
Q
Cumulative effect of HT1/3 but not HT2/3. Care to hypothesise why?
A
The expression of HT2 could have been great enough that the tag sequestered available antibody.
