Respiratory Processes Defined by Electron Donor Flashcards

1
Q

What type of molecule serves as the electron donor for chemoorgano(hetero)trophs?

A

Organic molecules

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2
Q

What type of molecule serves as the electron donor for chemolitho(auto)trophs?

A

Inorganic molecules

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3
Q

What is the major difference between aerobic and anaerobic respiration?

A

Aerobic respiration uses oxygen as the terminal electron acceptor, while anaerobic respiration uses other electron acceptors.

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4
Q

What is the major differences between anaerobic respiration and fermentation?

A

Anaerobic respiration uses an electron transport chain, while fermentation does not.

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5
Q

Why did aerobic respiration become the dominant form of respiration on Earth?

A

It is more efficient in producing ATP compared to anaerobic processes.

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6
Q

Why are anoxic habitats still widely spread in nature? (2 reasons)

A
  1. They provide niches for specialized organisms. 2. They are formed in environments with limited oxygen availability.
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7
Q

Why are organisms that use more electronegative electron acceptors typically obligate anaerobes?

A

They cannot survive in the presence of oxygen, which is toxic to them.

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8
Q

What happens to nitrate, sulfate, and carbon dioxide during assimilative reduction?

A

They are converted into organic compounds.

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9
Q

What happens to nitrate, sulfate, and carbon dioxide during dissimilative reduction?

A

They are reduced to gases or other inorganic compounds.

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10
Q

What are the major differences between assimilative and dissimilative reductions?

A

Assimilative reduction incorporates substrates into biomass, while dissimilative reduction does not.

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11
Q

What is a mixotroph?

A

An organism that can use both organic and inorganic compounds for energy.

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12
Q

The reactions of what trophic group of microbes form the heart of the major nutrient cycles?

A

Decomposers

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13
Q

What is the overall chemical reaction used by aerobic ‘hydrogen bacteria’ to produce ATP? What enzyme catalyzes this reaction?

A

2H2 + O2 → 2H2O

Catalyzed by hydrogenase.

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14
Q

What is the source of most of the hydrogen that is oxidized by hydrogen bacteria?

A

Hydrogen gas produced by anaerobic processes.

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15
Q

Some hydrogen bacteria make two different hydrogenase enzymes. What advantage do these bacteria have over ones that only make a membrane-bound hydrogenase?

A

They can utilize hydrogen more efficiently under varying environmental conditions.

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16
Q

Most hydrogen bacteria are facultative chemolithotrophs. What does this mean?

A

They can switch between using inorganic compounds and organic compounds for energy.

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17
Q

What method is used by most hydrogen bacteria to fix carbon when growing autotrophically?

A

Calvin cycle

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18
Q

Why do oxic environments typically contain very low levels of H2? (2 reasons)

A
  1. H2 is rapidly oxidized. 2. Oxygen is a more favorable electron acceptor.
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19
Q

What are the four most common sulfur compounds used as electron donors?

A

Sulfide, thiosulfate, sulfate, and elemental sulfur.

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20
Q

In most cases, what is the final product of sulfur-compound oxidation?

21
Q

The first step of sulfide oxidation produces what substance? How is this substance ‘stored’ by bacterial cells?

A

Sulfite

Stored as intracellular granules.

22
Q

Why have many sulfur bacteria evolved to be acid-tolerant or acidophilic?

A

To thrive in acidic environments where they can outcompete other organisms.

23
Q

What is the Sox system?

A

A biochemical pathway for the oxidation of sulfur compounds.

24
Q

Elements of the Sox system have been found in what two trophic groups of prokaryotes? How do these two groups differ in their use of sulfur compound oxidation?

A

Chemolithotrophs and phototrophs; chemolithotrophs use sulfur compounds for energy, while phototrophs use light.

25
What is the key enzyme of the Sox system and what is its function?
SoxB; it catalyzes the oxidation of sulfite to sulfate.
26
Electrons removed from sulfur compounds can be used for what two purposes?
1. ATP production. 2. Reducing power for biosynthesis.
27
Most sulfur chemolithotrophs use _______ as the terminal electron acceptor but some can use _______.
Oxygen; nitrate
28
Why do acidophilic chemolithotrophic 'iron bacteria' need to oxidize large amounts of iron to survive?
To obtain sufficient energy for growth in acidic conditions.
29
What is rusticyanin, where in the cell is it located, and what is its function?
A copper-containing protein located in the periplasm; it transfers electrons from iron to the electron transport chain.
30
What type of respiration is performed by acidophilic chemolithotrophic iron oxidizers?
Aerobic respiration
31
Why can’t acidophilic chemolithotrophic iron oxidizers use the large proton gradient that exists between the extracellular environment and the cytoplasm as a PMF to drive ATP synthesis?
The gradient is not sufficient due to the extreme acidity.
32
Certain chemolithotrophs and some phototrophic purple and green bacteria can oxidize iron in neutral pH environments. What advantage do these bacteria have over the acidophilic iron oxidizers?
They can thrive in a wider range of environmental conditions.
33
Why must neutrophilic iron oxidation occur under anaerobic conditions?
Oxygen inhibits the oxidation process.
34
Why is it more advantageous to oxidize FeS than Fe2+?
FeS oxidation yields more energy.
35
What exactly is nitrification?
The biological oxidation of ammonia to nitrate.
36
What part of the nitrification process is performed by 'ammonia oxidizers?'
The conversion of ammonia to nitrite.
37
What part of the nitrification process is performed by 'nitrite oxidizers?'
The conversion of nitrite to nitrate.
38
What is unique about certain members of the genus Nitrospira?
They can oxidize nitrite to nitrate without producing nitrous oxide.
39
What type of respiration is performed by nitrifying bacteria?
Aerobic respiration
40
Why do most species of the chemolithotrophic nitrite oxidizers also grow chemoorganotrophically, while the ammonia oxidizers are either obligate chemolithotrophs or mixotrophs?
Nitrite oxidizers can utilize organic carbon sources, while ammonia oxidizers are more specialized.
41
What important ecological roles are performed by nitrifying bacteria?
They convert ammonia into nitrate, facilitating nutrient cycling.
42
What is the anammox chemical reaction?
NH4+ + NO2- → N2 + 2H2O
43
What is unusual about the members of the Planctomycetes phylum of bacteria?
They have complex cell structures, including membrane-bound organelles.
44
What are anammoxosomes?
Specialized organelles in anammox bacteria that contain the enzymes for the anammox process.
45
How is the structure of an anammoxosome different from typical membranous organelles like those found in eukaryotic cells? Why is this structure necessary?
Anammoxosomes lack a typical membrane and are surrounded by a lipid bilayer; this structure is necessary to create a unique environment for the anammox reaction.
46
Nitrite is used by anammox bacteria in what two ways?
1. As a substrate for the anammox reaction. 2. As an electron acceptor.
47
In nature, where do anammox bacteria get their nitrite?
From the oxidation of ammonia by nitrifying bacteria.
48
How can aerobic ammonia oxidizers coexist with anaerobic ammonia oxidizers?
They occupy different niches and utilize different environmental conditions.
49
What important ecological roles are performed by anammox bacteria?
They contribute to nitrogen removal in wastewater treatment and play a role in the nitrogen cycle.