Respiration - Chapter 12 Flashcards
What are the 4 stages in respiration ?
- Glycolysis
- The Link reaction
- The Krebs cycle
- Oxidative phosphorylation
What is glycolysis ?
The splitting of glucose
What does respiration produce?
ATP
Where does glycolysis occur?
In the cytoplasm
Describe the process of glycolysis.
- phosphorylation of glucose using ATP, making triose phosphate
- Oxidation of triose phosphate to pyruvate (removal of hydrogen from triose phosphate for oxidation)
- net gain of ATP (2 ATP)
- NAD goes to reduced NAD
The process of glycolysis (detailed version)
-phosphorylating glucose to glucose phosphate using ATP
(The inorganic phosphates come from the hydrolysis of 2 molecules of ATP)
- each glucose molecule is split into 2 molecules of a 3 carbon compound : triose phosphate
- oxidation of triose phosphate to produce pyruvate
-hydrogen is removed from each triose phosphate molecule and transferred to NAD forming reduced NAD (NADH)
- a series of enzyme controlled reactions convert TP molecules to 3 Pyruvate molecules regenerating 2 ATP molecules from ADP
What are the products of glycolysis?
2x pyruvate (used in the link reaction)
Net gain of 2 ATP ( although there is 4, 2 had to be used for glucose phosphate)
2x NADH (will be used in oxidative phosphorylation)
What type of process is glycolysis ?
It is an aerobic process (occurs in both aerobic and anaerobic processes)
Where does the link reaction take place
In the mitochondrial matrix
What type of process is the link reaction ?
An aerobic process
In the absence of oxygen, anaerobic respiration takes place in the _____
If oxygen is present, then the cell carries out aerobic respiration in ________
Cytoplasm
Mitochondria
What is the reaction in the link reaction ?
Pyruvate + coenzyme A ~~~~~~~~~~~~~~~~> acetyl coenzyme A + CO2
NAD~> reduced NAD
Link reaction doesn’t require oxygen
Explain the link reaction ?
-pyruvate and NADH are diffuse from the cytoplasm into the mitochondrial matrix
- the pyruvate made in glycolysis is oxidised to acetate
-acetate combines with coenzyme A to produce acetyl coenzyme A
-the remaining one carbon part of the pyruvate (see diagram) leaves as a molecule of carbon dioxide
-at the same time,an oxidation reaction takes place NAD + H ~>reduced NAD (NADH)
-during a link reaction, a molecule of CO2 is released from the pyruvate. When CO2 is removed from the molecule, it’s called decarboxylation reaction
Because we have an oxidation + decarboxylation reaction, this is called oxidative decarboxylation
When is there a decarboxylation reaction in the link reaction, and then is there an oxidation reaction ?
During a link reaction, a molecule of CO2 is released from the pyruvate. When CO2 is removed from a molecule, it’s called a decarboxylation reaction.
Oxidation reaction - NAD + H ~> reduced NAD (NADH)
How many times does the link reaction take place for each molecule of glucose entering respiration and why ?
Glycolysis produces 2 pyruvate molecules for each molecule of glucose.
So the link reaction takes place twice for each molecule of glucose entering respiration ( as it only uses 1 pyruvate molecule in the reaction).