Respiration - A12 Flashcards

1
Q

What are the sub-cellular structures in the mitochondria?

A

Matrix, outer membrane(phospholipid bilayer), inner membrane(phospholipid bilayer), cristae(folds), ribosomes, mitochondrial DNA

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2
Q

What is a coenzyme?

A

a molecule that aids the function of an enzyme by transferring a chemical group from one molecule to another

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3
Q

What coenzymes are involved in respiration?

A

-NAD and FAD(used in transferring hydrogen)
-coenzyme A(transfers acetate between molecules)

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4
Q

What is the equation for respiration?

A

Word:
Glucose+oxygen->carbon dioxide+water+ATP
Symbol:
C6H12O6+6O2->6CO2+6H2O+32ATP

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5
Q

What reactants are needed for ATP synthesis?

A

ADP+Pi, water

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6
Q

What products are made in ATP Synthesis?

A

ATP

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7
Q

What type of reaction is ATP synthesis?

A

condensation

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8
Q

What enzyme is used in ATP Synthesis?

A

ATP synthase

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9
Q

What reactants are needed in ATP breakdown?

A

ATP

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10
Q

What products are made in ATP breakdown?

A

ADP+Pi, water

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11
Q

What type of reaction is ATP breakdown?

A

hydrolysis

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12
Q

What enzyme is used in ATP breakdown?

A

ATP hydrolase

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13
Q

What are the properties of ATP?

A

ATP is a good source of energy because:
-stores and releases small, manageable amounts of energy at a time - none wasted as heat
-small and soluble so can be transported around the cell
-it’s hydrolysed in a single step reaction

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14
Q

What is ATP used for?

A

-transporting substances across membranes
-anabolic reactions
-movement
-maintaining body temp.

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15
Q

What is needed in glycolysis?

A

1 glucose
2 ATP
2 ADP
2 NAD

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16
Q

What is produced in glycolysis?

A

2 pyruvate
4 ATP - net 2 ATP
2 NADH

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17
Q

What is needed in the link reaction?

A

1 pyruvate
1 NAD
1 coenzyme A

18
Q

What is produced in the link reaction?

A

1 NADH
1 CO2
1 acetyl coA

19
Q

How many times does the link reaction and Krebs cycle happen?

20
Q

What is needed in the Krebs cycle?

A

1 acetyl coA
3 NAD
1 FAD
1 ADP+Pi

21
Q

What is produced in the Krebs cycle?

A

2 CO2
3 NADH
1 FADH
1 ATP - net 2 ATP

22
Q

What is used in oxidation phosphorylation?

A

10 NADH
2 FADH
6 O2

23
Q

What is produced in oxidation phosphorylation?

A

10 NAD
2 FAD
6 H2O

24
Q

What is needed in total for respiration?

A

1 glucose
6 oxygen

25
What is produced in total for respiration?
6 carbon dioxide 6 water 32 ATP
26
Where does glycolysis occur?
cytoplasm
27
Describe the process of glycolysis.
-Glucose is phosphorylated using a phosphate from a molecule of ATP so glucose is more reactive -produces glucose phosphate and ADP -ATP is used to add another phosphate, forming glucose bisphosphate(hexose) -This is split into two triose phosphates -The triose phosphates are oxidised forming two molecules of pyruvate -NAD collects the hydrogen ions, forming 2 NADH -4 ATP are produces, but two were used in phosphorylation, so there is a net gain of two ATP
28
Where does the link reaction occur?
mitochondrial matrix
29
Describe the link reaction.
-pyruvate is decarboxylated, so one carbon atom is removed from pyruvate in the form of carbon dioxide -at the same time, pyruvate is oxidised to form acetate and NAD is reduced to for NADH -acetate is then combined with coenzyme A to form acetyl coenzyme A -no ATP is produced
30
Where does the Krebs cycle occur?
mitochondrial matrix
31
Describe the Krebs cycle.
-Acetyl CoA(2 carbons) combines with oxaloacetate(4 carbons) to form citrate(6 carbon compound) -Coenzyme A goes back to the link reaction to be used again -Citrate is converted into a 5 carbon intermediate compound through decarboxylation and oxidation(and dehydrogenated)(NAD is reduced to NADH) -the 5 carbon compound is converted into oxaloacetate(4 carbon compound) -it is decarboxylated and oxidised(dehydrogenated), producing FADH from FAD and 2 NADH from 2 NAD, as well as CO2 -ATP is produced by the direct transfer of a phosphate group from an intermediate molecule to ADP
32
What is substrate phosphorylation?
when a phosphate group is directly transferred from one molecule to another
33
Where does oxidative phosphorylation occur?
Inner membrane
34
Describe the process of oxidative phosphorylation.
1)Hydrogen atoms are released from NADH and FADH as they are oxidised to NAD and FAD. The hydrogen atoms split into protons(H+) and electrons(e-). 2)the electrons move down the electron transfer chain, losing energy at each carrier 3)the energy is used by the electron carriers to pump protons from the mitochondrial matrix into the intermembrane space(between the inner and outer mitochondrial membranes) 4)The conc. of protons is now higher in the intermembrane space than in the matrix-electrochemical gradient has been formed. 5)Protons move down the electrochemical gradient, back down across the inner mitochondrial membrane and into the matrix via ATP synthase(which is embedded in the inner mitochondrial membrane). This movement drives the synthesis of ATP from ADP and Pi. 6)This process of ATP production driven by the movement of H+ ions across a membrane(due to electrons moving down an electron transport chain) is called chemiosmosis. 7)In the matrix, at the end of the transport chain, the protons, electrons and oxygen(from the blood) combine to water. Oxygen is said to be the final electron acceptor.
35
What stage is involved in anaerobic respiration and what is produced?
One involves the first stage, glycolysis and produces lactic acid or ethanol and CO2.
36
What is pyruvate converted to in anaerobic respiration?
-ethanol through alcoholic fermentation in yeast and plants -lactate through lactate fermentation in animals and some bacteria -both use reduced NAD
37
What happens after the production of lactate or ethanol that allows biological processes to continue?
-production of lactate or ethanol regenerates NAD -therefore glycolysis can continue even when there isn’t much oxygen available -so a small amount of ATP can still be produced to keep some biological processes going
38
What is yeast?
A single celled organism that can be grown in a culture. It produces cO2 in either type of respiration
39
How can yeast be used to measure respiration rate?
Rate of CO2 production indicates respiration rate. CO2 production can be measured by using a gas syringe to collect O2.
40
What are respirometers used for?
-can be used to indicate the rate of aerobic respiration by measuring the volume of oxygen consumed by an organism over a period of time -can be used for small animals such as wood lice and also seeds/plants