Respiration Flashcards
reactions of aerobic respiration
Glycolysis
Link reaction
Krebs cycle
Respiratory Chain Reactions
main purpose of respiration
release energy from food so that ATP can be synthesized in a series of chemical reactions that completely oxidize glucose molecules
where does glycolysis take place?
in the cytoplasm of the cell without oxygen
phosphorylation reactions in glycolysis
glucose is oxidized to glucose-6-phosphate and further phosphorylated to fructose bizphosphate
what happens for each glucose molecule that is phosphorylated?
2 ATP molecules are split to release inorganic phosphate and energy for the process of phosphorylation
what is the purpose of phosphorylation of glucose?
to activate thhe glucose so that it is more likely to participate in glycolysis
to make it impossible for glucose to leave the cell since the cell membrane is impermeable to glucose-6-phosphate and fructose bizphosphate
what is produced during the splitting of fructose bizphosphate?
3C sugar phosphate molecules are produced
isomers must be in the form of triose phosphate in order to be metabolized
what is NAD?
a hydrogen acceptor/ carrier
what does the triose phosphate molecule undergo?
dehydrogenation in which 2H atoms are removed and taken up by the hydrogen acceptor, NAD, which becomes reduced to NADH and H+
the triose phosphate becomes oxidized to an intermediate compound which is eventually converted to glycerate-3-phosphate (substrate level phosphorylation occurs to incorporate a high energy phosphate molecule from one of the intermediated to produce a molecule of ATP)
how is phosphenol pyruvate produced?
through a series of reactions involving glycerate-3-phosphate
how is pyruvate produced?
phosphenol pyruvate is converted in a series of steps to a molecule of pyruvate and a high energy phosphate molecule is removed
what is produced altogether from each glucose molecule in glycolysis
2 ATP molecules are used for phosphorylation
4 ATP molecules are manufactured by substrate level phosphorylation
2 reduced NAD molecules
2 pyruvate molecules are manufactured
net production of ATP is 2 per glucose molecule
what does the reduced NAD do in glycolysis?
transports the H atoms into the mitochondria to the respiratory chain where phosphorylation will occur
structure of the mitochondria
spherical in shape
outer membrane is not very permeable but contains transport proteins that allow pyruvate to enter from the cytoplasm
inner membrane is foldedand contains many proton pumps and actively carries protons to the inter membranal space
many electron transport and channel proteins to which ATP synthase is attached
intermembranal space of mitochondria
high concentration of protons which are important for maintaining an electrochemical gradient
matrix of mitochondria
gel like in consistency
contains enzymes and chemicals needed for the link reaction and the krebs cycle reactions
has a suitable pH for enzyme activity
contains phosphate granules
70s ribosomes and circular DNA molecule
where does the link reaction occur?
in the matrix of the mitochondria