Respiration Flashcards
The roles of the coenzymes and carrier proteins in the synthesis of atp (4)
reduced nad, h+ions or, and electrons transferred in a series of redox reactions. energy is made available as electrons passed on, energy used to synthesise atp from adp and phosphate. protons passed into intermembrane space
atp is formed in the mitochondria by (6)
substrate level phosphorylation, the krebs cycle produces reduced coenzymes and electrons are released from these reduced co enzymes and pass along the etc. releases energy. allows protons to enter the intermembrane space, protons re enter the matrix via atp synthase, along the proton gradient. atp forms from adp+pi and oxygen is the final electron acceptor.
the enzyme that catalyses isocitrate to a-ketaglutorate is (2)
dehydrogenase, a h+ is removed from isocitrate
suggest how you could determine the dry mass of a sample of plant material
ibtain a sample of plant material and heat to remove water. ensure its below 100 degrees to ensure it doesnt combust, re measure its mass at regular intervals until the mass is constant.
advantage of using dry not fresh mass
as diff plants have diff water contents so this woudl change the mass ratio formed
suggest how a student could investigate the effects of a named variable on the rate of resp of a single celled organism
use a respirometer and use a dye as the terminal electron acceptor for the etc
what is the purpose of the sodium hydroxide solution in a resp set up to measure the rate of aerobic resp
absorbs co2 so that there is a net decrease in pressure as 02 is consumed
Explain why converting pyruvate to lactate allows the continued production of
ATP during anaerobic respiration.
it allows the co enzyme reduced nad to be re oxidised to ensure that glycolysis can continue.
Explain the line between P and Q
No aerobic respiration
- (Because) no (respiratory) substrate
Explain the line between Q and R.
(Oxygen concentration falls because)
1. Aerobic respiration ( have to say aerobic) uses oxygen
- Oxygen is terminal / electron acceptor
For the first 10 minutes, the tap attached to tube A was left open and the syringe
from tube B was removed.
Suggest three reasons why the apparatus was left for 10 minutes.
Equilibrium reached.
Allow for expansion / pressure change in apparatus;
Allow respiration rate of seeds to stabilise.
Suggest and explain why the chosen temperature was 20 °C for this experiment.
Optimum temperature for normal growth of seeds;
2. (Optimum temperature) for enzymes involved in respiration.
Suggest why the rate of gas production decreased between 50 and 60
minutes.
GLUCOSe decreases
) Yeast can also respire aerobically. The student repeated the investigation with
a fresh sample of yeast in glucose solution, but without the oil. All other
conditions remained the same.
Explain what would happen to the volume of gas in the syringe if the yeast
were only respiring aerobically.
(Stays the) same level
2. Same volume of oxygen uptake and carbon dioxide
release;
why does inhibiting the proton gradient mean that more heat was produced and why does this mean that the uptake of oxygen remained constant.
heat released from electron transort chain energy not used to produce atp so released as heat. oxygen used as final electron acceptor.
explain the advantages of collecting a large number of results
increases reliability of averages and can identify anomalies.
name 2 substances formed from pyruvate
co2, water, atp etc
describe how acetyl coa is formed in the link reac
oxidation of pyruvate AND co2 is released, addition of coa
ocalocetae is the first substrate to bind to blah blah explain how it binds
change in shape of active site, moulds around enzyme. complementary to substrate
suggest how the production of succinyl coa could control the rate of reac catalysed by citrate synthase
compeitive inhibitor, prevenets escs forming
the structure of malanoate is very similar to succinate. the scientists added malonate and the resp substrate pyruvate to a suspension of isolated mitochondria. explain why the scientist did not use glucose as the resp substrate for these isolated mitochondria
glucose is used during glycolysis, it cannot cross mitochondrial membranes
explain how malonate inhibits the formation of fumarate from succinate
competitive inhibitor, prevents escs forming
the scientist measured the uptake of oxygen by the mitochondria during the investigation. the uptake of oxygen decreased when malonate was added, why
krebs cycle inhibited as nad not reduced, hydrogens not passed to etc so oxygen cannot be used as the final electron acceptor.
suggesr one explanation for the higher glycerol and fatty acid concentrations in the blood plasma of the athletes after they were given caffeine
breakdown of fats;
at increased rate
explain why the rq for glucose is 1
idea that volumes of oxygen and carbon dioxide the same;