Research Lecture 4 - Kayte Jenkin

Question 1

Education background

Answer 1

• bachelor science (biomedical) & bachelor psychology (2005-2008)
• honours in biomedical science (2009)
• doctorate of phiosophy, PhD (2010-2014)

Question 2

research background

Answer 2

• renal and intestinal physiology

- cell culture and small animal models are used to investigate.

Question 3

NHE3 mediated intestinal fluid absorption

Answer 3

• sodium hydrogen exchanger 3 (NHE3) is responsible for the electroneutral sodium uptake from the intestine
• two projects: LPA as a treatment to increase activity of NHW 3 and comparative physiology

Question 4

Cannabinoid

Answer 4

• receptors are localised to the cell membrane, but can also be internalised
• when bound to ligand, the receptor couples with G proteins which initiates a signalling cascade within the target cell
• CB receptors are capable of biased signalling: signalling cascade initiated may differ dependiing on the ligand which binds to receptor

Question 5

Endocannabinoid system and disease- CB1 receptor

Answer 5

• predominantly expressed in the CNS: appetite, learning and memory, anxiety, depression, schizophrenia, stroke, MS, neurogeneration, epilepsy and addiction
• also found in peripheral tissues: pain, energy metabolism, atherosclerosis, inflammination, glaucoma, cancer, GI, liver, kidney and musculoskeletal disorders

Question 6

Endocannabinoid system and disease- CB2 receptor

Answer 6

• CB2 is highly expressed in immune cells: inflammation, rheumatoid arthritis, colitis, crohns disease, MS, pain pathways
• also found in CNS and peripheral tissues: artherosclerosis, Alzheimer’s kidney, liver musculoskeletal disorders

Question 7

Pathogenesis of CKD

Answer 7

• initially hypertrophy (inc cell size)and hyperplasia (inc cell no.) is observed
• as disease progresses, inflammation, fibrosis (tubules and glomerulus_ podocyte loss and accumulation of the extracellular matrix occurs