Required Practical Flashcards
What is the independent and dependant variable of the experiment testing the pH level effect on enzymes?
The independent variable is the pH level, while the dependent variable is the enzyme activity or reaction rate.
What are the sources of error for the experiment that tests the effect of pH level on enzymes?
The sources of error could be contamination, measurement errors, and temperature changes. pH levels could also vary slightly from the target level due to buffer limitations.
What could the uncontrolled variables be in the experiment that tests the effect of pH level on enzymes?
Uncontrolled variables could include temperature, enzyme concentration, substrate concentration, and agitation rate.
How could you improve the experiment that tests the effect of pH level on enzymes?
The experiment can be improved by controlling temperature, enzyme and substrate concentration, and agitation rate. The use of a buffer solution can help maintain the pH level, and repeated trials can be conducted to reduce random error.
What is the conclusion of the experiment that tests the effect of pH level on enzymes?
The results of the experiment indicate that enzymes have an optimum pH range where their activity is highest. As the pH deviates from this range, enzyme activity decreases, with extreme pH levels resulting in denaturation of the enzyme.
How do you carry out rate calculations for the experiment that tests the effect of pH level on enzymes?
The rate of enzyme activity can be calculated by dividing the change in concentration of the product (or substrate) over time. For example, if the concentration of product increases from 0.2 M to 0.8 M over 60 seconds, the rate of reaction would be (0.8 - 0.2)/60 = 0.01 M/s.
