Required Practical 11

Question 1

Describe how a calibration curve could be produced for glucose

Answer 1

• use distilled water and glucose solution of known concentration to produce a dilution series
• heat a set volume of each solution with a set volume of Benedict’s solution
• measure absorbance using a colorimeter
• plot graph with absorbance on y axis against concentration of glucose solution on x axis

Question 2

How can the concentration of glucose in an unknown urine sample be identified?

Answer 2

• perform Benedict’s test using same volumes of solutions used in producing calibration curve
• measure absorbance using a colorimeter
• read of calibration curve to find glucose concentration