Required Practical 1 Flashcards
What are the 4 factors that affect enzyme activity?
- Enzyme Concentration
- Substrate concentration
- Temperature
- pH
How is a control set up in a practical measuring enzyme activity?
Replace the enzyme solution with distilled water or boiled enzyme solution
How can the results of the practical measuring enzyme activity be used to find the initial rate of reaction?
- Plot your results on a graph of ‘rate of reaction’ against ‘time’
- Draw a tangent at time t=0 to find the initial rate
How is the rate of reaction calculated from time?
Rate of reaction= 1/time
What of the effect of temperature on enzyme activity?
- As temperature increases, kinetic energy increases so more ES complexes form. The rate of reaction increases increases up to the optimum temperature
- Beyond that, bonds in the enzyme tertiary structure break, which changes the shape of the active site. The substrate and enzyme are no longer complementary, so rate of reaction decreases
What is the risk and level of risk associated with handling enzymes?
Students may have allergic reactions to enzymes, so avoid contact with skin and eyes, wear eye protection
Explain why buffers are used
To maintain the pH
You left the test tubes in the water bath for 10 minutes before you added the enzyme to the milk powder solutions. Explain why
To let it heat up to the temperature
Describe and explain what you did to make sure the temperatures of the water baths were as reliable as possible
Add a thermometer and check it was at a constant temperature
Name two control variables for this experiment
- Temperature of water bath
- Concentration of enzyme/substrate