Required Practical 1 Flashcards

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1
Q

What are the 4 factors that affect enzyme activity?

A
  • Enzyme Concentration
  • Substrate concentration
  • Temperature
  • pH
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2
Q

How is a control set up in a practical measuring enzyme activity?

A

Replace the enzyme solution with distilled water or boiled enzyme solution

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3
Q

How can the results of the practical measuring enzyme activity be used to find the initial rate of reaction?

A
  • Plot your results on a graph of ‘rate of reaction’ against ‘time’
  • Draw a tangent at time t=0 to find the initial rate
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4
Q

How is the rate of reaction calculated from time?

A

Rate of reaction= 1/time

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5
Q

What of the effect of temperature on enzyme activity?

A
  • As temperature increases, kinetic energy increases so more ES complexes form. The rate of reaction increases increases up to the optimum temperature
  • Beyond that, bonds in the enzyme tertiary structure break, which changes the shape of the active site. The substrate and enzyme are no longer complementary, so rate of reaction decreases
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6
Q

What is the risk and level of risk associated with handling enzymes?

A

Students may have allergic reactions to enzymes, so avoid contact with skin and eyes, wear eye protection

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7
Q

Explain why buffers are used

A

To maintain the pH

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8
Q

You left the test tubes in the water bath for 10 minutes before you added the enzyme to the milk powder solutions. Explain why

A

To let it heat up to the temperature

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9
Q

Describe and explain what you did to make sure the temperatures of the water baths were as reliable as possible

A

Add a thermometer and check it was at a constant temperature

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10
Q

Name two control variables for this experiment

A
  • Temperature of water bath
  • Concentration of enzyme/substrate
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