Replication Of HIV-1

Question 0

Name the receptors and tropisms of HIV-1

Answer 0

T-cell tropic strain: CD4 molecule and CXCR 4 chemokine receptor

Macrophage tropic strain: CD4 molecule and CCR5 chemokine receptor

Question 1

What is the retrovirus genome? Other than lentivirus

Answer 1

GAG
POL
ENV

Question 2

Describe the reverse transcriptase mechanism

Answer 2

RNA is used as a template to make a single stranded DNA genome.

In RNA-DNA hybrid, RNA is dissolved leaving the single stranded DNA genome

Single stranded DNA copy is used to make double stranded DNA copy (provirus)

Question 3

What is the job of integrase for the proviral DNA?

Answer 3

Binds the 2 ends of the proviral DNA and brings them together and in close proximity to the cellular DNA

It takes off the 3’ end of the proviral DNA and is inserted into the host DNA

The host enzyme carries out repair

Question 4

What increases the level of transcription of the HIV provirus?

Answer 4

TAT

Question 5

What elevates the virus REPLICATION?

Answer 5

Nef