Regulation of gene expression Flashcards
1
Q
Cis
A
The promoter sequence is cis acting on lac Z
Control gene expression of genes on the same piece of dna
2
Q
Trans
A
Control gene expression on other dna molecules
The repressor protein is trans acting on lac Z
3
Q
Lacl
A
Repressor gene - encodes the repressor
It trans acting and is dominant
4
Q
Lacl^S
A
Produces a super repressor that does not bind lactose
It is trans dominant and binds both operaors preventing transcription in the presence or absence of lactose
5
Q
LacOc mutation
A
Mutation of the operator
Is constitutive
Prevents repressor from binding to it
LacO gene is cis acting
B-galactosidase is produced when lactose is present or absent bc the lacOc is paired with lac Z
6
Q
If we have lacOc and lacZ-
A
A non functional B galactose daze is produced
