References

Question 1

Ott et al 2008

Answer 1

Decellularised heart with intact 3D geometry and vasculature was repopulated with ECs

Made a contractile mouse myocardium

Question 2

Langer et al 2004

Answer 2

Describes the history of biomaterials

Properties of a good biomaterial

Question 3

Halstenberg et al 2014

Answer 3

Biomimetic polymer with lysine and lactic acid

Allowed amino acid coupling reactions to take place without affecting the biocompatibility of the polymer

Question 4

Sanwinki et al 2008

Answer 4

Dichotomous participation of stem cells with breast cancer

82 women

Proportion of microchimeric women with breast cancer was lower than those without

Question 5

Roach et al 2016

Answer 5

Importance of curvature on nanoscale

Question 6

Kaplan et al 2011

Answer 6

Phototropism

Question 7

Discher et al 2005

Answer 7

Cells probe elasticity as they pull on their surroundings

With increased stiffness, cells change their cytoskeleton and adhesions

Question 8

Wiley et al 1998

Answer 8

Vroman effect

Question 9

Drury et al 2009

Answer 9

Cells attach to surfaces without protein via nonspecific forces

But all biological processes happen in a protein dependent manner

Surface chemistry is changed to determine how proteins attach

Question 10

Langer et al 2004

Answer 10

Tailor scaffolds to be biomimetic to the original tissue

Example is modifying through adding PEG

Reduces non-specific effects of protein adsorption

Increases the circulating time of the biomaterial

Question 11

Song et al 2013

Answer 11

Carried out decellularisation of a rat kidney

Recellualrisation and in vitro kidney function

Worse function than native healthy kidney - possibly due to vein immaturity of regenerated kidney

Question 12

Song et al 2013 - criticisms

Answer 12

Used <10%

There is an increase in collagen in the decellularised organ

This means they are comparing dry and wet weights, cannot trust their DNA measurements

Question 13

Zhang et al 2006

Answer 13

Change topography on nanoscale have improved personalisation of scaffolds

Question 14

Definition of TERM by Langer and Vicanti in 1993

Answer 14

Interdisciplinary field of research that applied the principles of engineering and processes and phenomena of the life sciences towards the development of biological substitutes that restore, maintain or improve tissue function

Question 15

Definition of TERM by Dr Williams in 2010

Answer 15

Do not need scaffolds

Question 16

Yuan et al 2003

Answer 16

Cobalt chloride and HIF stabilisation

Question 17

Buga et al 2014

Answer 17

Role of PDGF and VEGF in angiogenesis at different time points of angiogenesis

Question 18

Bar et al 2010

Answer 18

Venous and tubular structures require EC repopulations to generate a functional vascular network

To increase adhesions of these cells, CNN-1 was bound to the matrix surface to improve re-endothelization