Red and White Cells Flashcards

1
Q

What value do you use to interpret the leukogram?

A

absolute count

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2
Q

When do you need to correct the white cell count due to nRBCs?

A

when nRBCs are > 10

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3
Q

Primary site for hematopoiesis after birth?

A

bone marrow

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4
Q

Release of white cells from the maturation/storage pool is ______

A

orderly

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5
Q

What “pool” are the majority of the white cells found in?

A

maturation/storage

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6
Q

Endothelial cells produce _____ while neutrophils produce ______?

A

1) selectins

2) integrins

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7
Q

Digestion by lysosomal enzyme is considered oxygen-________

A

independent

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8
Q

Neutrophils _____ reenter circulation while lymphocytes ______ reenter circulation

A

1) cannot

2) can

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9
Q

A stimulus for an increase in neutrophils usually leads to an increase in __________ as well. Why?

A

Monocytes; share a common stem cell

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10
Q

What mediates physiologic leukocytosis?

A

catecholamine release

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11
Q

What mediates stress leukograms?

A

corticosteroids

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12
Q

When is the left shift significant?

1) cats and dogs
2) cows and horses

A

1) >1000 bands

2) >300 bands

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13
Q

mild, moderate, marked, extreme leukocytosis in dogs and cats (ranges)

A

Mild– up to 25,000
moderate– 25,000-50,000
Marked– 50,000-70,000
extreme– >70,000

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14
Q

Mild, significant, marked leukocytosis in horses and cattle

A

mild– up to 15,000
significant– 15,000-25,000
marked– >25,000

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15
Q

What does the plasma protein: fibrinogen ratio correct for?

A

dehydration

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16
Q

major area of Epo production?

A

kidney peritubular cells

17
Q

What is the major determinant of blood viscosity?

A

Deformability

18
Q

What state does Fe need to be in to bind O2?

A

Fe2+ (ferrous)

19
Q

What enzyme keeps Fe in the reduced state for hgb synthesis?

A

Methemoglobin reductase

20
Q

Rate limiting step of heme synthesis?

A

Delta-ALA-synthase (1st enzyme)

21
Q

what molecule binds free iron in the blood

A

apotransferrin

22
Q

mutations of what two enzymes can alter RBC metabolism?

A

PK (pyruvate kinase)

PFK (phosphofructokinase)

23
Q

What are two damage control pathways for RBCs?

A

Methemoglobin reduction pathway (NADH)

Glutathione (NADPH)

24
Q

Two scavenger molecules of intravascular hemolysis?

A

Haptoglobin and hemopexin

25
Q

What is MCV? What are it’s descriptive terms?

A

mean corpuscular voume

micro, normo, macro, –CYTIC

26
Q

What is MCHC? descriptive terms?

A

mean corpuscular hgb concentration

hypo, normo, hyper –CHROMIC

27
Q

A regenerative anemia will often appear as ______ and ______

A

macrocytic

hypochromic

28
Q

T/F: Polychromasia is usually seen in horses

A

False

29
Q

What RBC measurement is the best way to evaluate bone marrow response to anemia?

A

reticulocyte count

30
Q

Why do we use CRP?

A

to correct for the severity of anemia

31
Q

In which spp. do we use RPI and why?

A

Dogs; corrects CRP for reticulocyte lifespan in circulation

32
Q

What regulates Fe uptake from enterocytes?

A

Ferroportin

33
Q

What molecule regulates iron uptake based on need?

A

Hepcidin

34
Q

Which storage form of iron is most rapidly mobilized for use?

A

Ferritin

35
Q

2 ways inflammatory cytokines suppress erythropoiesis

A

1) decrease Epo production and responsiveness

2) reduce availability of Fe for Hgb synthesis

36
Q

Two types of absolute erythrocytosis. Relation to Epo for each?

A

1) Primary– independent of Epo

2) secondary– increased Epo

37
Q

Appropriate vs. Inappropriate seconday absolute erythrocytosis?

A

Appropriate–in response to tissue hypoxia

Inappropriate–independent of tissue oxygenation