Recombinant DNA Technology Flashcards
1
Q
what does recombinant DNA technology involve?
A
- transfer of DNA fragments from one organism or species to another
2
Q
what is universal as well as the genetic code?
A
- transcription and translation mechanisms
3
Q
how can fragments of DNA be produced?
A
- conversion of mRNA to complementary DNA using reverse transcriptase
- using restriction endonucleases
- creating a gene in the gene machine
4
Q
explain the method of recombinant DNA technology using reverse transcriptase.
A
- mRNA is isolated from cells
- mRNA is added to free DNA nucleotides and reverse transcriptase
- reverse transcriptase uses mRNA as a template to synthesis cDNA
5
Q
explain the method of recombinant DNA technology using restriction endonuclease.
A
- section of DNA have palidromic sequences of nucleotides(antiparallel base pairs - AGGT and TGGA)
- restriction endonucleases recognise specific palindromic sequences so cut DNA at these places
6
Q
why are there different restriction endonucleases?
A
- different restriction endonucleases cut at different specific recognition sequences, shape of recognition sequence is complementary to the enzyme active site
7
Q
what can be left when using restriction endonucleases?
A
- sticky ends
- unpaired bases at the end of a fragment
8
Q
how can sticky ends be used?
A
anneal DNA fragments to another DNA fragment that has sticky ends with complementary sequences
9
Q
how can gene machines be used?
A
10
Q
where can DNA fragments be amplified?
A
- in vitro cloning
- in vivo cloning
11
Q
A
