Recombinant DNA

Question 1

Characteristics Restriction Enzyme

Answer 1

Restriction enzyme hydrolyzes the phosphodiester bond of each strand . Have two types of cutting pattern which is staggered cut and straight cut . Produce two type of ends which is sticky ends and blunt ends

Question 2

Characteristics DNA cloning vector

Answer 2

Able to accept foreign DNA in multiple cloning site (MCS) . Able to replicate freely in the host cell . Has selectable marker gene

Question 3

Characteristics host cell

Answer 3

Able to accept recombinant DNA through transformation process . Able to maintain structure of recombinant DNA from one generation to another . Able to express the gene of the recombinant DNA

Question 4

Characteristics modifying enzyme

Answer 4

DNA ligase join the separate DNA strand together by catalyzing the re-formation of phosphodiester bonds between them . Forms a recombinant DNA . Taq polymerase is a modifying enzyme . Isolated from bacteria living in hot springs . A heat stable type of DNA polymerase