recombinant DNA

Question 1

Define a vector in molecular cloning

Answer 1

• Vector is a DNA molecule used to carry foreign genetic material in another cell where it can be replicated.

Question 2

what are 4 recombinant vectors?

Answer 2

• plasmids
• bacteriophages
• viruses
• artificial chromosomes

Question 3

Where are plasmids found and how are they transformed?

Answer 3

• found in bacteria

- transferred by transformation and conjugation

Question 4

What is an example of a bacteriophage vector and what do they transfer?

Answer 4

• Lambda (found in bacteria)

- transfer of antimicrobial resistance through mechanism called transduction

Question 5

What are 2 types of virus vectors?

Answer 5

1. non-primate lentiviruses - vectors used to integrate DNA in mamalian cells.
2. baculoviruses - vectors used in combination with recombinant expression in insect cells (a eukaryotic system)