Receptor Flashcards
14564341: which human chromosome all three cytokine s
5
14564341:where are the receptors
pseudoautosomal regions of short arm of X-Y chromosome: IL-3 and GM-CSFRa
Chromosome 3: IL-5Ra
Chromosome 22: Common beta chain
14564341: 3 pathways down stream
JAK-STAT
MAPK (ERK, JNK p38)
PI3-Kinase
14564341: what are the cells in nature paper
mast cells and basophils (infection induced)
14564341: what is contrasting in GM-CSF vs IL-3 for DC polarization
GM-CSF for Th1 and IL-3 for Th2 promoting DCs from human monocytes
11207369: how many subunits in a dodecameric protein
12 (note: it is a quarternary structure look at last figure)
12055233: GM-CSF alpha chain CD number
CD116
12055233: human monocytes GM-CSF vs. IL-3
less IL-12p70 and IL-1beta and more IL-10: with IL-3 compared to GM-CSF
8543766: CD34+ CD33 low express
most IL-3 RA (but not useful since it is just one phase of the general lineage and not necessarily indicates any cell lineage)
9155834: asthma and GM-CSF R
mostly on intrinsic but not on atopic asthma
9600506: Fc(epsilon)RI-high dendritic cells express high levels of
CD123 (basophils also do)
19250840: plasmacytoid DC fraction in PBMC
0.2 to 0.8% human PBMC
19250840: what brings pDC to infected skin
chemerin (chemoattractant made by fibroblasts and endothelial cells)
18641647: what type of CPG ODN stimulate pDC
type A which causes pDC to make IFNa which then stimulate NK cells
Type B makes pDC to make IL-6 and TNF (good for antibody production)
18641647: full form of CpG ODN
oligo deoxy nucloetide
Cytidine guanosine sequence
TLR9
7579364: neutrophils express IL-3R after pre-Rx with
GM-CSF
8100720: where are the receptors
GM-CSF and IL-3 both in in the PAR of X and Y
12743324: Bone marrow contains - times more than peripheral blood of pDC
5 times
wiki: origin of follicular DC
mesenchymal (they look like DC but not from BM)
22930820: parasite induced IL-3 dependent - in BM and spleen
basophilia
Sepsis science paper: highest IL-3R seen in
GMP: granulocyte macrophage
MDP: macrophage and dendiritc
16174104: In human T cell clones, IL-3 and IL-13 was resultant of
GATA-3 (looks good)
8934648: endothelial cells express IL-3R but not-
GMCSF R
10527461: IL-3R expression in hematopoetic stem cells that are also positive for
CD34 (taken from different tissues) correlates with growth potential
23226269: in brain IL-3R expressed in neural progenitors and neurons in the neonatal period
also correlates with brain volume
8976174: apprently a dichotoomy of IL-3 and GM-CSF occurs in differentiation of
mDC and pDC
11739152: surprise! pDC marker and growth in mice
B220 and CD11c+ Thy1.2+
NO proliferate but diffeentiate with IL-3 and anti-CD40
Note that they are FLT3 treated
16174108 : pDC marker
CD11cint, CD4+/–, IL-3R– B220+ CD45RA+
Miltenyi: pDC marker
mPDCA1 (BST-2)
Siglec H
CD11c low
CD11bNeg
11017101: Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent TH1 polarization
in humans
25137390: IL-3 beta chain can bind to IL-3 without alpha but —
can’t do signaling
1582416: Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3).
gives a schematic
19281803: mental disese that is connected to IL-3R
schizophrenia
14730712:autocrine activator in brain
microglia (main source is also microglia)
8806599: Expression of IL-3 receptor in testis.
Leydig cells
7727767: C57BL/6 and BALB/c expresses correct
IL-3Ra (many lab strains don’t, but wild do)
Lack (11713464) or less (12438422) CD123 on pDC
15254595 (but Mellor shows expression)
