RDNA Technology Flashcards
To know steps in rdna technology, tools needed and definition of tools
1
Q
What are the steps for RDNA Technology?
A
Isolation of desired gene by using restriction enzymes which cuts DNA at specific sites.
Next, using the same restriction enzyme ,the plasmid linearized by being cleaved, creating a site for the insertion of the desired gene. Thus, obtaining a recombinant plasmid
Thereafter, the plasmid is introduced to a bacteria cell, through a process called transformation.
Finally, the bacteria cell divides and produces the desired genes
2
Q
Tools needed for RDNA Technology?
A
- Plasmids
- Restriction enzymes - Enzymes that cut DNA
- Gel electrophoresis
- Polymerase Chain Reaction (PCR)
3
Q
Processes needed in RDNA Technology?
A
- Linearization of plasmid (cutting of plasmid using the same restriction enzyme)
- Ligation
- Transformation (Introduction of recombinant plasmid to a host organism)
4
Q
Function of restriction enzyme
A
- To cut DNA at specific sites
5
Q
What is a vector?
A
It is a plasmid that is used to transfer DNA into a living cell
5
Q
What is a plasmid?
A
A small circular DNA used to transfer DNA into a living cell
