RBC Preservation

Question 1

Measure of in vivo RBC survival following transfusion

Answer 1

RBC viability

Question 2

The loss of RBC Viability has been correlated with the ____________, which is associated with biochemical changes

Answer 2

Storage lesion

Question 3

RBC Storage Lesions (Decreased Values)

Answer 3

Viable cells (%)
Glucose
ATP
pH
2,3-dpg

Question 4

RBC Storage Lesions (Increased Values)

Answer 4

Lactic Acid
Plasma K+
Plasma Hemoglobin

Question 4

How much iron is contained in one RBC unit?

Answer 4

220-250 mg of iron

Question 4

An anticoagulant preservative wherein adenine is incorporated into the CPD solution which increases ADP levels, thereby driving glycolysis towards the synthesis of ATP

Answer 4

CPDA-1

Question 4

Storage time of anticoagulants

ACD-A
CPD
CP2D
CPDA-1

Answer 4

ACD-A, CPD, CP2D = 21 days
CPDA-1 = 35 days

Question 4

CPDA-1 components

Answer 4

0.25 mM of Adenine
25% more glucose than CPD

Question 5

Majority of blood is stored in what type of plastic bags?

Answer 5

Polyvinyl chloride (PVC) plastic bags

Question 9

PVC bags related to the plasticizer _____________ which is used in the manufacture of bags and has been found to leach from the plastic into the lipids of the plasma medium and RBC membranes of the blood during storage. It also has a tendency to break at low temperatures, therefore components frozen in PVC bags must be handled with care

Answer 9

di(ethylhexyl)-phthalate(DEHP)

Question 10

Chemical in Anticoagulant Solutions

Chelates calcium; preventing clotting

Answer 10

Citrate (sodium citrate/citric acid)

Question 11

Chemicals in Anticoagulant Solutions

Maintains pH during storage; necessary for maintenance of adequate levels of 2,3-DPG

Answer 11

Monobasic sodium phosphate

Question 12

Chemicals in Anticoagulant Solutions

Substrate for ATP production (cellular energy)

Answer 12

Dextrose

Question 12

Chemicals in Anticoagulant Solutions

Production of ATP (Extends shelf-life from 21 to 35 days)

Answer 12

Adenine