RBC and Platelet Preservation

Question 1

How many blood and anticoagulant-preservative solution are contained for EACH UNIT of collected whole blood from donor

Answer 1

450 mL blood and 63 mL anticoag-preservative sol.
500 mL blood and 70 mL anticoag-preservative sol.

Question 2

When can a donor give blood (intervals)?

Answer 2

Every 8 weeks

Question 3

How many screening tests are done to samples from donors for each unit of donated blood?

Answer 3

10 (infectious disease markers)

Question 4

How many percent post-transfusion survival of RBCs is necessary for a successful transfusion?

Answer 4

75%

Question 5

Enumerate the approved preservative solutions for storage of RBCs

Answer 5

ACD, CPD, CP2D, CPDA-1

Question 6

Storage temp. and days of ACD?

Answer 6

1-6 degrees Celsius; 21 days

Question 7

Storage temp. and days of CPD?

Answer 7

1-6 degrees Celsius; 21 days

Question 8

Storage temp. and days of CP2D?

Answer 8

1-6 degrees Celsius; 21 days

Question 9

Storage temp. and days of CPDA-1

Answer 9

1-6 degrees Celsius (not sure); 35 days

Question 10

RBC additive sol. approved in the US?

Answer 10

Adsol, Nitrucel, Optisol

Question 11

Number of days for RBC storage using the additive solutions approved in the US?

Answer 11

42 days

Question 12

Additive solutions are appropriate for what patient population?

Answer 12

Neonates and pediatric

Question 13

RBCs have been traditionally glycerolized and frozen within 6 days of whole blood collection in what preservative solution?

Answer 13

CPD and CPDA-1

Question 14

For how many years does a glycerolized and frozen within 6 days collected whole blood be stored in CPD and CPDA-1 preservatives?

Answer 14

10 years

Question 15

The only FDA-approved rejuvenation solution used in blood centers to regenerated ATP and 2,3-DPG levels before freezing?

Answer 15

Rejuvesol

Question 16

This is used to salvage O-type and rare RBC units that are outdated or with specific anticoag-preservative sol. up to 3 days past outdate?

Answer 16

Rejuvenation

Question 17

Measured after transfusion to determine whether the platelets in the body of the recipient are viable

Answer 17

Corrected Count Increment (CCI)

Question 18

Storage limit of platelets

Answer 18

5 days

Question 19

Platelets are stored at what temp?

Answer 19

Room temp (22 degrees Celsius) ; in the book, 20-24 degrees Celsius

Question 20

Why does platelet needs constant agitation?

Answer 20

They are prone to clumping
They adhere to the surface of the blood bag
They aggregate together

Question 21

What are the two main reasons for the 5-day shelf-life for platelets

Answer 21

Bacterial contamination at incubation of 22 degrees Celsius
Platelet storage lesion

Question 22

Platelet storage lesion: Lactate

Answer 22

Increased

Question 23

Platelet storage lesion: pH

Answer 23

Decreased

Question 24

Platelet storage lesion: ATP

Answer 24

Decreased

Question 25

Platelet storage lesion: Morphology scores change from discoid to spherical

Answer 25

Decreased

Question 26

Platelet storage lesion: Degranulation

Answer 26

Increased

Question 27

Platelet storage lesion: Platelet activation markers

Answer 27

Increased

Question 28

After 5 days, platelet concentration cannot be used

Answer 28

True

Question 29

This facilitate oxygen transfer into the platelet bag and oxygen consumption by the platelets

Answer 29

Agitation

Question 30

pH for platelet storage

Answer 30

6.2-6.8