Rapid Molecular Drug Tests

Question 1

What are genktypic tests abd examples

Answer 1

Naat 
Amplification of targeted gebetic region by pcr
Xpert tb- detect and rr
Xpert mdr/xdr
True nat mtb- chip based 
Line probe assay

Question 2

Line probe assay moa

Answer 2

Use pcr and reverse hybridization techniques for detection of mutation

Question 3

Types of lpA

Answer 3

First line- rr and inh
Second line - fq- gyr a and gye b gene
Rrs and eis- low level kanamycin resistance

Question 4

Phenotypic

Answer 4

Cultures
Growth based
Bactec mgit 960- automated liquid culture system
Uses oxygen quencher for flouriscence detection as agrowtb index

Question 5

Xpert mdr/xdr detevts mutations of which drugs

Moa

Answer 5

Inh , fq, sli , eto in single test
Semiquantitative nested pcr f/b high resolutiom melt technology
Inh- oxyr- aphc intergenic region , fab g

Question 6

What is TAT for
Lj
Liquid
Molecular- lpa. Cbnaat, trunat

Answer 6

3 , 4-8wks
.8-10 days , 2-6 wks
1-3 days
2hrs
2hrs- 1hr for detection  and 1hr for resistance